Rac1 is required for cell proliferation and G2/M progression.


Journal Article

We have transiently expressed a dominant negative form of rac1 (N17rac1) using adenoviral-mediated gene transfer. The level of N17rac1 expression is demonstrated to be proportional to the multiplicity of infection. Expression of N17rac1 in Rat 2 fibroblasts results in cytostatic growth arrest. Cell-cycle analysis demonstrates that cells expressing N17rac1 accumulate in G2/M. These results suggest that rac1 is required for cell proliferation and provide the first demonstration in mammalian cells of a role for small GTP-binding proteins in the G2/M transition.

Full Text

Cited Authors

  • Moore, KA; Sethi, R; Doanes, AM; Johnson, TM; Pracyk, JB; Kirby, M; Irani, K; Goldschmidt-Clermont, PJ; Finkel, T

Published Date

  • August 1997

Published In

Volume / Issue

  • 326 ( Pt 1) /

Start / End Page

  • 17 - 20

PubMed ID

  • 9337845

Pubmed Central ID

  • 9337845

Electronic International Standard Serial Number (EISSN)

  • 1470-8728

International Standard Serial Number (ISSN)

  • 0264-6021

Digital Object Identifier (DOI)

  • 10.1042/bj3260017


  • eng