Serodiagnosis of La Crosse virus infections in humans by detection of immunoglobulin M class antibodies.


Journal Article

Sera from 92 humans with illnesses clinically compatible with those caused by California serogroup virus infections were tested for antibody to La Crosse (LAC) virus by using the immunoglobulin M (IgM) antibody-capture enzyme-linked immunosorbent assay (MAC ELISA), the IgG ELISA, and the hemagglutination inhibition (HI), complement fixation and serum dilution-plaque reduction neutralization tests. On the reported day of onset of illness in 18 individuals, 94% had IgM antibody, 50% had neutralization antibody, 33% had HI antibody, and 11% had IgG antibody. Neutralization, HI, and IgG antibody prevalence rates increased thereafter, whereas IgM antibody prevalence remained high (92% 2 or more weeks after the onset of illness). It was concluded that the MAC ELISA is a sensitive test for the presence of antibody to LAC virus. The sensitivity of the MAC ELISA and the rapidity with which it can be performed appear to provide a powerful tool for the clinically relevant serodiagnosis of LAC virus infections in humans.

Full Text

Duke Authors

Cited Authors

  • Calisher, CH; Pretzman, CI; Muth, DJ; Parsons, MA; Peterson, ED

Published Date

  • April 1986

Published In

Volume / Issue

  • 23 / 4

Start / End Page

  • 667 - 671

PubMed ID

  • 3700625

Pubmed Central ID

  • 3700625

International Standard Serial Number (ISSN)

  • 0095-1137


  • eng

Conference Location

  • United States