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A role for Ni in the hormonal stimulation of adenylate cyclase.

Publication ,  Journal Article
Cerione, RA; Staniszewski, C; Caron, MG; Lefkowitz, RJ; Codina, J; Birnbaumer, L
Published in: Nature
November 21, 1985

The best understood system for transduction of extracellular messages into intracellular signals is the hormone receptor-coupled adenylate cyclase. In such systems receptors are functionally coupled to the enzyme by two special proteins, termed the stimulatory and inhibitory guanine nucleotide regulatory proteins (Ns and Ni, respectively). These proteins, thought to mediate, respectively, stimulatory and inhibitory influences on the adenylate cyclase, are members of a larger class of heterotrimeric guanine nucleotide regulatory proteins involved in membrane signal transduction. We have studied the interactions of the various components of the adenylate cyclase system by co-reconstituting pure beta-adrenergic receptors, pure Ns and Ni, and functionally resolved preparations of the catalyst in phospholipid vesicles. In the absence of Ni, beta-adrenergic receptor/Ns-mediated catecholamine stimulation of the enzyme is relatively modest (approximately 1.3-fold). Surprisingly, however, when Ni is also present, stimulation increases dramatically (up to 7-8-fold) because of a greater suppression of basal relative to agonist-stimulated enzyme activity. Thus, Ni may actually be required for maximal agonist stimulation as well as for inhibition of the adenylate cyclase.

Duke Scholars

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

November 21, 1985

Volume

318

Issue

6043

Start / End Page

293 / 295

Location

England

Related Subject Headings

  • Receptors, Adrenergic, beta
  • Isoproterenol
  • Humans
  • General Science & Technology
  • GTP-Binding Proteins
  • Dose-Response Relationship, Drug
  • Adenylyl Cyclases
 

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Cerione, R. A., Staniszewski, C., Caron, M. G., Lefkowitz, R. J., Codina, J., & Birnbaumer, L. (1985). A role for Ni in the hormonal stimulation of adenylate cyclase. Nature, 318(6043), 293–295. https://doi.org/10.1038/318293a0
Cerione, R. A., C. Staniszewski, M. G. Caron, R. J. Lefkowitz, J. Codina, and L. Birnbaumer. “A role for Ni in the hormonal stimulation of adenylate cyclase.Nature 318, no. 6043 (November 21, 1985): 293–95. https://doi.org/10.1038/318293a0.
Cerione RA, Staniszewski C, Caron MG, Lefkowitz RJ, Codina J, Birnbaumer L. A role for Ni in the hormonal stimulation of adenylate cyclase. Nature. 1985 Nov 21;318(6043):293–5.
Cerione, R. A., et al. “A role for Ni in the hormonal stimulation of adenylate cyclase.Nature, vol. 318, no. 6043, Nov. 1985, pp. 293–95. Pubmed, doi:10.1038/318293a0.
Cerione RA, Staniszewski C, Caron MG, Lefkowitz RJ, Codina J, Birnbaumer L. A role for Ni in the hormonal stimulation of adenylate cyclase. Nature. 1985 Nov 21;318(6043):293–295.
Journal cover image

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

November 21, 1985

Volume

318

Issue

6043

Start / End Page

293 / 295

Location

England

Related Subject Headings

  • Receptors, Adrenergic, beta
  • Isoproterenol
  • Humans
  • General Science & Technology
  • GTP-Binding Proteins
  • Dose-Response Relationship, Drug
  • Adenylyl Cyclases