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Cloning of the cDNA for the human beta 1-adrenergic receptor.

Publication ,  Journal Article
Frielle, T; Collins, S; Daniel, KW; Caron, MG; Lefkowitz, RJ; Kobilka, BK
Published in: Proc Natl Acad Sci U S A
November 1987

Screening of a human placenta lambda gt11 library has led to the isolation of the cDNA for the human beta 1-adrenergic receptor (beta 1AR). Used as the probe was the human genomic clone termed G-21. This clone, which contains an intronless gene for a putative receptor, was previously isolated by virtue of its cross hybridization with the human beta 2-adrenergic receptor (beta 2AR). The 2.4-kilobase cDNA for the human beta 1AR encodes a protein of 477 amino acid residues that is 69% homologous with the avian beta AR but only 54% homologous with the human beta 2AR. This suggests that the avian gene encoding beta AR and the human gene encoding beta 1AR evolved from a common ancestral gene. RNA blot analysis indicates a message of 2.5 kilobases in rat tissues, with a pattern of tissue distribution consistent with beta 1AR binding. This pattern is quite distinct from the pattern obtained when the beta 2AR cDNA is used as a probe. Expression of receptor protein in Xenopus laevis oocytes conveys adenylate cyclase responsiveness to catecholamines with a typical beta 1AR specificity. This contrasts with the typical beta 2 subtype specificity observed when the human beta 2AR cDNA is expressed in this system. Mammalian beta 1AR and beta 2AR are thus products of distinct genes, both of which are apparently related to the putative G-21 receptor.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 1987

Volume

84

Issue

22

Start / End Page

7920 / 7924

Location

United States

Related Subject Headings

  • Turkeys
  • Sequence Homology, Nucleic Acid
  • Receptors, Adrenergic, beta
  • Molecular Sequence Data
  • Humans
  • DNA, Recombinant
  • DNA
  • Base Sequence
  • Animals
  • Amino Acid Sequence
 

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Frielle, T., Collins, S., Daniel, K. W., Caron, M. G., Lefkowitz, R. J., & Kobilka, B. K. (1987). Cloning of the cDNA for the human beta 1-adrenergic receptor. Proc Natl Acad Sci U S A, 84(22), 7920–7924. https://doi.org/10.1073/pnas.84.22.7920
Frielle, T., S. Collins, K. W. Daniel, M. G. Caron, R. J. Lefkowitz, and B. K. Kobilka. “Cloning of the cDNA for the human beta 1-adrenergic receptor.Proc Natl Acad Sci U S A 84, no. 22 (November 1987): 7920–24. https://doi.org/10.1073/pnas.84.22.7920.
Frielle T, Collins S, Daniel KW, Caron MG, Lefkowitz RJ, Kobilka BK. Cloning of the cDNA for the human beta 1-adrenergic receptor. Proc Natl Acad Sci U S A. 1987 Nov;84(22):7920–4.
Frielle, T., et al. “Cloning of the cDNA for the human beta 1-adrenergic receptor.Proc Natl Acad Sci U S A, vol. 84, no. 22, Nov. 1987, pp. 7920–24. Pubmed, doi:10.1073/pnas.84.22.7920.
Frielle T, Collins S, Daniel KW, Caron MG, Lefkowitz RJ, Kobilka BK. Cloning of the cDNA for the human beta 1-adrenergic receptor. Proc Natl Acad Sci U S A. 1987 Nov;84(22):7920–7924.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 1987

Volume

84

Issue

22

Start / End Page

7920 / 7924

Location

United States

Related Subject Headings

  • Turkeys
  • Sequence Homology, Nucleic Acid
  • Receptors, Adrenergic, beta
  • Molecular Sequence Data
  • Humans
  • DNA, Recombinant
  • DNA
  • Base Sequence
  • Animals
  • Amino Acid Sequence