Molecular characterization of dopamine receptors.
Published
Journal Article (Review)
The D1 and D2 dopamine receptors have been biochemically characterized using specific probes based on the subtype selective antagonists SCH 23390 and spiperone, respectively. The D2 dopamine receptor was identified from several tissues by photoaffinity labeling and was purified from bovine anterior pituitary to homogeneity using a combination of affinity, lectin and hydroxylapatite chromatography. A complementary DNA (cDNA) encoding a rat brain D2 dopamine receptor has been cloned via low stringency hybridization using a portion of the beta 2-adrenergic receptor gene as a probe. Photoaffinity crosslinking and affinity chromatography have also been used to identify and purify the rat brain D1 dopamine receptor.
Full Text
Duke Authors
Cited Authors
- Bates, MD; Gingrich, JA; Bunzow, JR; Falardeau, P; Dearry, A; Senogles, SE; Civelli, O; Caron, MG
Published Date
- June 1, 1990
Published In
Volume / Issue
- 3 / 6 Pt 2
Start / End Page
- 29S - 33S
PubMed ID
- 2143386
Pubmed Central ID
- 2143386
International Standard Serial Number (ISSN)
- 0895-7061
Digital Object Identifier (DOI)
- 10.1093/ajh/3.6.29s
Language
- eng
Conference Location
- United States