Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit.

Published

Journal Article

To facilitate functional and mechanistic studies of receptor-G protein interactions, [corrected] the human beta 2-adrenergic receptor (h beta-AR) has been expressed in Saccharomyces cerevisiae. This was achieved by placing a modified h beta-AR gene under control of the galactose-inducible GAL1 promoter. After induction by galactose, functional h beta-AR was expressed at a concentration several hundred times as great as that found in any human tissue. As determined from competitive ligand binding experiments, h beta-AR expressed in yeast displayed characteristic affinities, specificity, and stereoselectivity. Partial activation of the yeast pheromone response pathway by beta-adrenergic receptor agonists was achieved in cells coexpressing h beta-AR and a mammalian G protein (Gs) alpha subunit-demonstrating that these components can couple to each other and to downstream effectors when expressed in yeast. This in vivo reconstitution system provides a new approach for examining ligand binding and G protein coupling to cell surface receptors.

Full Text

Duke Authors

Cited Authors

  • King, K; Dohlman, HG; Thorner, J; Caron, MG; Lefkowitz, RJ

Published Date

  • October 5, 1990

Published In

Volume / Issue

  • 250 / 4977

Start / End Page

  • 121 - 123

PubMed ID

  • 2171146

Pubmed Central ID

  • 2171146

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.2171146

Language

  • eng

Conference Location

  • United States