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Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit.

Publication ,  Journal Article
King, K; Dohlman, HG; Thorner, J; Caron, MG; Lefkowitz, RJ
Published in: Science
October 5, 1990

To facilitate functional and mechanistic studies of receptor-G protein interactions, [corrected] the human beta 2-adrenergic receptor (h beta-AR) has been expressed in Saccharomyces cerevisiae. This was achieved by placing a modified h beta-AR gene under control of the galactose-inducible GAL1 promoter. After induction by galactose, functional h beta-AR was expressed at a concentration several hundred times as great as that found in any human tissue. As determined from competitive ligand binding experiments, h beta-AR expressed in yeast displayed characteristic affinities, specificity, and stereoselectivity. Partial activation of the yeast pheromone response pathway by beta-adrenergic receptor agonists was achieved in cells coexpressing h beta-AR and a mammalian G protein (Gs) alpha subunit-demonstrating that these components can couple to each other and to downstream effectors when expressed in yeast. This in vivo reconstitution system provides a new approach for examining ligand binding and G protein coupling to cell surface receptors.

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Published In

Science

DOI

ISSN

0036-8075

Publication Date

October 5, 1990

Volume

250

Issue

4977

Start / End Page

121 / 123

Location

United States

Related Subject Headings

  • Signal Transduction
  • Saccharomyces cerevisiae
  • Restriction Mapping
  • Recombinant Fusion Proteins
  • Receptors, Adrenergic, beta
  • Promoter Regions, Genetic
  • Plasmids
  • Pindolol
  • Molecular Sequence Data
  • Macromolecular Substances
 

Citation

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King, K., Dohlman, H. G., Thorner, J., Caron, M. G., & Lefkowitz, R. J. (1990). Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit. Science, 250(4977), 121–123. https://doi.org/10.1126/science.2171146
King, K., H. G. Dohlman, J. Thorner, M. G. Caron, and R. J. Lefkowitz. “Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit.Science 250, no. 4977 (October 5, 1990): 121–23. https://doi.org/10.1126/science.2171146.
King K, Dohlman HG, Thorner J, Caron MG, Lefkowitz RJ. Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit. Science. 1990 Oct 5;250(4977):121–3.
King, K., et al. “Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit.Science, vol. 250, no. 4977, Oct. 1990, pp. 121–23. Pubmed, doi:10.1126/science.2171146.
King K, Dohlman HG, Thorner J, Caron MG, Lefkowitz RJ. Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit. Science. 1990 Oct 5;250(4977):121–123.
Journal cover image

Published In

Science

DOI

ISSN

0036-8075

Publication Date

October 5, 1990

Volume

250

Issue

4977

Start / End Page

121 / 123

Location

United States

Related Subject Headings

  • Signal Transduction
  • Saccharomyces cerevisiae
  • Restriction Mapping
  • Recombinant Fusion Proteins
  • Receptors, Adrenergic, beta
  • Promoter Regions, Genetic
  • Plasmids
  • Pindolol
  • Molecular Sequence Data
  • Macromolecular Substances