Bone histomorphometric and biomechanical abnormalities in mice homozygous for deletion of the dopamine transporter gene.
Dopamine (DA) has been reported to have effects on calcium and phosphorus metabolism. The dopamine transporter (DAT) is believed to control the temporal and spatial activity of released DA by rapid uptake of the neurotransmitter into presynaptic terminals. We have evaluated the histologic and biomechanical properties of the skeleton in mice homozygous for deletion of the DA transporter gene (DAT) to help delineate the role of DA in bone biology. We have demonstrated that DAT-/-mice have reduced bone mass and strength. DAT-/- animals had shorter femur length and dry weight. Ash calcium content of the femur was 32% lower in the DAT-/- mice than in the wild-type animals. Cancellous bone volume in the proximal tibial metaphysis was significantly lower in the DAT-/- animals (p < 0.04). There was a 32% reduction in trabecular thickness (p = NS). For the vertebrae, cancellous bone volume was again lower in the DAT-/- animals compared with wild-type as a consequence of increased trabecular spacing (p < 0.05) and reduced trabecular number (p < 0.05). Cortical thickness and bone area in the femoral diaphysis were reduced in the DAT-/-animals. The ultimate bending load (femoral strength) for the DAT-/- mice was 30% lower than the wild-type mice (p = 0.004). Thus, deletion of the DAT gene results in deficiencies in skeletal structure and integrity.
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Related Subject Headings
- Phenotype
- Nerve Tissue Proteins
- Mice, Knockout
- Mice
- Membrane Transport Proteins
- Membrane Glycoproteins
- Homozygote
- Endocrinology & Metabolism
- Dopamine Plasma Membrane Transport Proteins
- Dopamine
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Phenotype
- Nerve Tissue Proteins
- Mice, Knockout
- Mice
- Membrane Transport Proteins
- Membrane Glycoproteins
- Homozygote
- Endocrinology & Metabolism
- Dopamine Plasma Membrane Transport Proteins
- Dopamine