Cloning and functional characterization of a cocaine-sensitive dopamine transporter.

Journal Article (Journal Article)

We report the cloning of a rat cDNA encoding a functional dopamine transporter. This cDNA, derived from an intron-containing gene, encodes a protein of 620 amino acids. Hydropathicity analysis of the protein sequence suggests the presence of 12 putative transmembrane domains. The protein displays considerable identity with transporters for noradrenaline and GABA (64 and 30%, respectively). Transient expression of the cDNA in COS7 cells directs the expression of dopamine uptake activity with appropriate pharmacology and in a sodium-dependent fashion. In situ hybridization reveals that the mRNA for this transporter is expressed in the substantia nigra and ventral tegmental area, regions that contain dopaminergic cell bodies.

Full Text

Duke Authors

Cited Authors

  • Giros, B; el Mestikawy, S; Bertrand, L; Caron, MG

Published Date

  • December 16, 1991

Published In

Volume / Issue

  • 295 / 1-3

Start / End Page

  • 149 - 154

PubMed ID

  • 1765147

International Standard Serial Number (ISSN)

  • 0014-5793

Digital Object Identifier (DOI)

  • 10.1016/0014-5793(91)81406-x


  • eng

Conference Location

  • England