Adrenergic receptor homologies in vertebrate and invertebrate species examined by DNA hybridization.
Journal Article (Journal Article)
The deduced protein sequences of the mammalian adrenergic receptors (ARs) suggest that these proteins have evolved by several ancient gene duplication events. To investigate in what species these events may have occurred DNA fragments encoding the family of adrenergic receptors from human (beta 1AR and alpha 2AR) and hamster (beta 2AR and alpha 1AR) were used to detect homologous sequences in other vertebrates, invertebrates and unicellular organisms by Southern blot hybridization analysis. Sequences homologous to hamster beta 2AR were detected in lower vertebrates, invertebrates and Dictyostelium, but not in yeast or bacteria. Within vertebrates, sequences strongly homologous to human beta 1AR and human platelet alpha 2AR were confined to the higher vertebrates only. In the invertebrates, only Drosophila contained sequences homologous to hamster alpha 1AR. Our results suggest that non-mammalian species may contain receptors homologous to the mammalian adrenergic receptors and that the sequences homologous to human beta 2AR have been the most strongly conserved.
Full Text
Duke Authors
Cited Authors
- Palacios, JM; O'Dowd, BF; Cotecchia, S; Hnatowich, M; Caron, MG; Lefkowitz, RJ
Published Date
- 1989
Published In
Volume / Issue
- 44 / 26
Start / End Page
- 2057 - 2065
PubMed ID
- 2545987
International Standard Serial Number (ISSN)
- 0024-3205
Digital Object Identifier (DOI)
- 10.1016/0024-3205(89)90352-4
Language
- eng
Conference Location
- Netherlands