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Molecular characterization of the beta-adrenergic receptor of frog erythrocytes.

Publication ,  Journal Article
Caron, MG; Shorr, RG; Lavin, TN; Lefkowitz, RJ
Published in: Metabolism
July 1982

The beta-adrenergic receptor of the frog erythrocyte has been solubilized in an active form with digitonin and purified by affinity chromatography and high performance liquid chromatography. Purified preparations contain a single band of iodinated protein of apparent Mr = 58,000. This peptide appears to represent the ligand binding subunit of the receptor since purified preparations bind ligands with the same beta-adrenergic specificity as the solubilized or membrane-bound receptor, display the same isoelectric point and similar sedimentation characteristics in sucrose density gradients. The same ligand binding subunit can also be identified in partially purified receptor preparations or in membranes by photoaffinity labelling or photodependent crosslinking of two radiolabelled beta-adrenergic antagonists, p-azidobenzylcarazolol and p-aminobenzylcarazolol.

Duke Scholars

Published In

Metabolism

DOI

ISSN

0026-0495

Publication Date

July 1982

Volume

31

Issue

7

Start / End Page

658 / 663

Location

United States

Related Subject Headings

  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic
  • Photochemistry
  • Erythrocytes
  • Endocrinology & Metabolism
  • Chromatography, High Pressure Liquid
  • Chemistry
  • Chemical Phenomena
  • Anura
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Caron, M. G., Shorr, R. G., Lavin, T. N., & Lefkowitz, R. J. (1982). Molecular characterization of the beta-adrenergic receptor of frog erythrocytes. Metabolism, 31(7), 658–663. https://doi.org/10.1016/0026-0495(82)90195-0
Caron, M. G., R. G. Shorr, T. N. Lavin, and R. J. Lefkowitz. “Molecular characterization of the beta-adrenergic receptor of frog erythrocytes.Metabolism 31, no. 7 (July 1982): 658–63. https://doi.org/10.1016/0026-0495(82)90195-0.
Caron MG, Shorr RG, Lavin TN, Lefkowitz RJ. Molecular characterization of the beta-adrenergic receptor of frog erythrocytes. Metabolism. 1982 Jul;31(7):658–63.
Caron, M. G., et al. “Molecular characterization of the beta-adrenergic receptor of frog erythrocytes.Metabolism, vol. 31, no. 7, July 1982, pp. 658–63. Pubmed, doi:10.1016/0026-0495(82)90195-0.
Caron MG, Shorr RG, Lavin TN, Lefkowitz RJ. Molecular characterization of the beta-adrenergic receptor of frog erythrocytes. Metabolism. 1982 Jul;31(7):658–663.
Journal cover image

Published In

Metabolism

DOI

ISSN

0026-0495

Publication Date

July 1982

Volume

31

Issue

7

Start / End Page

658 / 663

Location

United States

Related Subject Headings

  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic
  • Photochemistry
  • Erythrocytes
  • Endocrinology & Metabolism
  • Chromatography, High Pressure Liquid
  • Chemistry
  • Chemical Phenomena
  • Anura
  • Animals