Role of the sphingosine-1-phosphate receptor EDG-1 in PDGF-induced cell motility.

Published

Journal Article

EDG-1 is a heterotrimeric guanine nucleotide binding protein-coupled receptor (GPCR) for sphingosine-1-phosphate (SPP). Cell migration toward platelet-derived growth factor (PDGF), which stimulates sphingosine kinase and increases intracellular SPP, was dependent on expression of EDG-1. Deletion of edg-1 or inhibition of sphingosine kinase suppressed chemotaxis toward PDGF and also activation of the small guanosine triphosphatase Rac, which is essential for protrusion of lamellipodia and forward movement. Moreover, PDGF activated EDG-1, as measured by translocation of beta-arrestin and phosphorylation of EDG-1. Our results reveal a role for receptor cross-communication in which activation of a GPCR by a receptor tyrosine kinase is critical for cell motility.

Full Text

Duke Authors

Cited Authors

  • Hobson, JP; Rosenfeldt, HM; Barak, LS; Olivera, A; Poulton, S; Caron, MG; Milstien, S; Spiegel, S

Published Date

  • March 2, 2001

Published In

Volume / Issue

  • 291 / 5509

Start / End Page

  • 1800 - 1803

PubMed ID

  • 11230698

Pubmed Central ID

  • 11230698

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.1057559

Language

  • eng

Conference Location

  • United States