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Phorbol ester effects on alpha 1-adrenoceptor binding and phosphatidylinositol metabolism in cultured vascular smooth muscle cells.

Publication ,  Journal Article
Cotecchia, S; Leeb-Lundberg, LM; Hagen, PO; Lefkowitz, RJ; Caron, MG
Published in: Life Sci
December 23, 1985

Tumor promoting phorbol esters stimulate Ca++ phospholipid-dependent protein kinase C. It has been suggested that this enzyme regulates the functional properties of different cell membrane receptors. In this study we investigated the effect of phorbol esters on alpha 1-adrenoceptor binding and phosphatidylinositol metabolism in cultured smooth muscle cells derived from rabbit aorta. Treatment of these cells with biologically active phorbol esters for 15 min. to 2 hours caused a marked decrease of norepinephrine stimulation of inositol phospholipid metabolism and a 3 fold decrease in agonist affinity for 125I-HEAT binding to alpha 1-adrenoceptors in the intact smooth muscle cells. The ability of phorbol esters to modulate alpha 1-adrenoceptor responsiveness suggests that activation of protein kinase C may represent an important mechanism regulating alpha 1-adrenergic receptor functional properties.

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Published In

Life Sci

DOI

ISSN

0024-3205

Publication Date

December 23, 1985

Volume

37

Issue

25

Start / End Page

2389 / 2398

Location

Netherlands

Related Subject Headings

  • Tetralones
  • Tetradecanoylphorbol Acetate
  • Receptors, Adrenergic, alpha
  • Rabbits
  • Prazosin
  • Phosphatidylinositols
  • Phosphatidic Acids
  • Phorbol Esters
  • Phenethylamines
  • Pharmacology & Pharmacy
 

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Cotecchia, S., Leeb-Lundberg, L. M., Hagen, P. O., Lefkowitz, R. J., & Caron, M. G. (1985). Phorbol ester effects on alpha 1-adrenoceptor binding and phosphatidylinositol metabolism in cultured vascular smooth muscle cells. Life Sci, 37(25), 2389–2398. https://doi.org/10.1016/0024-3205(85)90106-7
Cotecchia, S., L. M. Leeb-Lundberg, P. O. Hagen, R. J. Lefkowitz, and M. G. Caron. “Phorbol ester effects on alpha 1-adrenoceptor binding and phosphatidylinositol metabolism in cultured vascular smooth muscle cells.Life Sci 37, no. 25 (December 23, 1985): 2389–98. https://doi.org/10.1016/0024-3205(85)90106-7.
Cotecchia S, Leeb-Lundberg LM, Hagen PO, Lefkowitz RJ, Caron MG. Phorbol ester effects on alpha 1-adrenoceptor binding and phosphatidylinositol metabolism in cultured vascular smooth muscle cells. Life Sci. 1985 Dec 23;37(25):2389–98.
Cotecchia, S., et al. “Phorbol ester effects on alpha 1-adrenoceptor binding and phosphatidylinositol metabolism in cultured vascular smooth muscle cells.Life Sci, vol. 37, no. 25, Dec. 1985, pp. 2389–98. Pubmed, doi:10.1016/0024-3205(85)90106-7.
Cotecchia S, Leeb-Lundberg LM, Hagen PO, Lefkowitz RJ, Caron MG. Phorbol ester effects on alpha 1-adrenoceptor binding and phosphatidylinositol metabolism in cultured vascular smooth muscle cells. Life Sci. 1985 Dec 23;37(25):2389–2398.
Journal cover image

Published In

Life Sci

DOI

ISSN

0024-3205

Publication Date

December 23, 1985

Volume

37

Issue

25

Start / End Page

2389 / 2398

Location

Netherlands

Related Subject Headings

  • Tetralones
  • Tetradecanoylphorbol Acetate
  • Receptors, Adrenergic, alpha
  • Rabbits
  • Prazosin
  • Phosphatidylinositols
  • Phosphatidic Acids
  • Phorbol Esters
  • Phenethylamines
  • Pharmacology & Pharmacy