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Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor.

Publication ,  Journal Article
Cotecchia, S; Schwinn, DA; Randall, RR; Lefkowitz, RJ; Caron, MG; Kobilka, BK
Published in: Proc Natl Acad Sci U S A
October 1988

The cDNA for the Syrian hamster alpha 1-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the receptor protein purified from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes a 515-residue polypeptide that shows the most sequence identity with the other adrenergic receptors and the putative protein product of the related clone G-21. Similarities with the muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells confirm that we have cloned the alpha 1-adrenergic receptor that couples to inositol phospholipid metabolism.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 1988

Volume

85

Issue

19

Start / End Page

7159 / 7163

Location

United States

Related Subject Headings

  • Receptors, Adrenergic, alpha
  • Phosphatidylinositols
  • Molecular Sequence Data
  • Gene Expression Regulation
  • GTP-Binding Proteins
  • DNA
  • Cricetinae
  • Cloning, Molecular
  • Base Sequence
  • Animals
 

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Cotecchia, S., Schwinn, D. A., Randall, R. R., Lefkowitz, R. J., Caron, M. G., & Kobilka, B. K. (1988). Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor. Proc Natl Acad Sci U S A, 85(19), 7159–7163. https://doi.org/10.1073/pnas.85.19.7159
Cotecchia, S., D. A. Schwinn, R. R. Randall, R. J. Lefkowitz, M. G. Caron, and B. K. Kobilka. “Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor.Proc Natl Acad Sci U S A 85, no. 19 (October 1988): 7159–63. https://doi.org/10.1073/pnas.85.19.7159.
Cotecchia S, Schwinn DA, Randall RR, Lefkowitz RJ, Caron MG, Kobilka BK. Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7159–63.
Cotecchia, S., et al. “Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor.Proc Natl Acad Sci U S A, vol. 85, no. 19, Oct. 1988, pp. 7159–63. Pubmed, doi:10.1073/pnas.85.19.7159.
Cotecchia S, Schwinn DA, Randall RR, Lefkowitz RJ, Caron MG, Kobilka BK. Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7159–7163.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 1988

Volume

85

Issue

19

Start / End Page

7159 / 7163

Location

United States

Related Subject Headings

  • Receptors, Adrenergic, alpha
  • Phosphatidylinositols
  • Molecular Sequence Data
  • Gene Expression Regulation
  • GTP-Binding Proteins
  • DNA
  • Cricetinae
  • Cloning, Molecular
  • Base Sequence
  • Animals