Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor.


Journal Article

The cDNA for the Syrian hamster alpha 1-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the receptor protein purified from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes a 515-residue polypeptide that shows the most sequence identity with the other adrenergic receptors and the putative protein product of the related clone G-21. Similarities with the muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells confirm that we have cloned the alpha 1-adrenergic receptor that couples to inositol phospholipid metabolism.

Full Text

Duke Authors

Cited Authors

  • Cotecchia, S; Schwinn, DA; Randall, RR; Lefkowitz, RJ; Caron, MG; Kobilka, BK

Published Date

  • October 1988

Published In

Volume / Issue

  • 85 / 19

Start / End Page

  • 7159 - 7163

PubMed ID

  • 2845398

Pubmed Central ID

  • 2845398

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.85.19.7159


  • eng

Conference Location

  • United States