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Mechanisms of hormone receptor-effector coupling: the beta-adrenergic receptor and adenylate cyclase.

Publication ,  Journal Article
Lefkowitz, RJ; Caron, MG; Michel, T; Stadel, JM
Published in: Fed Proc
August 1982

The beta-adrenergic receptors that are coupled to adenylate cyclase have provided a model system for studying the mechanisms by which a plasma membrane receptor is coupled to a well-defined biochemical effector. The beta 2-adrenergic receptors from frog erythrocyte membranes have been purified to homogeneity and the ligand-binding subunit has been identified as a glycoprotein with an approximate molecular weight of 58,000. This subunit has also been identified with the use of newly developed photoaffinity reagents. Under the influence of agonist hormones (H), the receptors (R) form transient complexes with another component of this system, termed the nucleotide regulatory protein (N). Formation of this ternary complex, HRN, leads to the dissociation of GDP from N and the interaction of stimulatory GTP with N. N charged with GTP appears to activate the catalytic moiety of the adenylate cyclase enzyme. Although some striking analogies have been found for the mechanisms by which inhibitory receptors interact with adenylate cyclase, much less is known about the molecular properties of the components involved and the ways in which they interact to dampen adenylate cyclase activity in the plasma membrane.

Duke Scholars

Published In

Fed Proc

ISSN

0014-9446

Publication Date

August 1982

Volume

41

Issue

10

Start / End Page

2664 / 2670

Location

United States

Related Subject Headings

  • Receptors, Cell Surface
  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic
  • Rats
  • Radioligand Assay
  • Models, Biological
  • Guanosine Triphosphate
  • GTP-Binding Proteins
  • Erythrocyte Membrane
  • Cyclic AMP
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lefkowitz, R. J., Caron, M. G., Michel, T., & Stadel, J. M. (1982). Mechanisms of hormone receptor-effector coupling: the beta-adrenergic receptor and adenylate cyclase. Fed Proc, 41(10), 2664–2670.
Lefkowitz, R. J., M. G. Caron, T. Michel, and J. M. Stadel. “Mechanisms of hormone receptor-effector coupling: the beta-adrenergic receptor and adenylate cyclase.Fed Proc 41, no. 10 (August 1982): 2664–70.
Lefkowitz RJ, Caron MG, Michel T, Stadel JM. Mechanisms of hormone receptor-effector coupling: the beta-adrenergic receptor and adenylate cyclase. Fed Proc. 1982 Aug;41(10):2664–70.
Lefkowitz, R. J., et al. “Mechanisms of hormone receptor-effector coupling: the beta-adrenergic receptor and adenylate cyclase.Fed Proc, vol. 41, no. 10, Aug. 1982, pp. 2664–70.
Lefkowitz RJ, Caron MG, Michel T, Stadel JM. Mechanisms of hormone receptor-effector coupling: the beta-adrenergic receptor and adenylate cyclase. Fed Proc. 1982 Aug;41(10):2664–2670.

Published In

Fed Proc

ISSN

0014-9446

Publication Date

August 1982

Volume

41

Issue

10

Start / End Page

2664 / 2670

Location

United States

Related Subject Headings

  • Receptors, Cell Surface
  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic
  • Rats
  • Radioligand Assay
  • Models, Biological
  • Guanosine Triphosphate
  • GTP-Binding Proteins
  • Erythrocyte Membrane
  • Cyclic AMP