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Pure beta-adrenergic receptor: the single polypeptide confers catecholamine responsiveness to adenylate cyclase.

Publication ,  Journal Article
Cerione, RA; Strulovici, B; Benovic, JL; Lefkowitz, RJ; Caron, MG
Published in: Nature
December 8, 1983

The beta-adrenergic receptor binding subunits from frog erythrocytes, hamster lung and guinea pig lung have been purified to apparent homogeneity and in all cases reside on a single polypeptide. Insertion of the pure receptors into phospholipid vesicles and subsequent fusion of these vesicles with a receptor-deficient cell conveys beta-adrenergic responsiveness to the adenylate cyclase system of the acceptor cell. Such responsiveness is linearly dependent on the amount of receptor used in the fusion experiments and is independent of the receptor source. Moreover, this responsiveness displays appropriate beta-adrenergic specificity. These results indicate that the beta-adrenergic receptor polypeptide contains both the ligand binding site and the site responsible for mediating stimulation of adenylate cyclase activity, presumably via interaction with the guanine nucleotide regulatory protein.

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Published In

Nature

DOI

ISSN

0028-0836

Publication Date

December 8, 1983

Volume

306

Issue

5943

Start / End Page

562 / 566

Location

England

Related Subject Headings

  • Xenopus laevis
  • Receptors, Adrenergic, beta
  • Membranes, Artificial
  • Lung
  • Isoproterenol
  • Guinea Pigs
  • General Science & Technology
  • Erythrocytes
  • Dose-Response Relationship, Drug
  • Cricetinae
 

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Cerione, R. A., Strulovici, B., Benovic, J. L., Lefkowitz, R. J., & Caron, M. G. (1983). Pure beta-adrenergic receptor: the single polypeptide confers catecholamine responsiveness to adenylate cyclase. Nature, 306(5943), 562–566. https://doi.org/10.1038/306562a0
Cerione, R. A., B. Strulovici, J. L. Benovic, R. J. Lefkowitz, and M. G. Caron. “Pure beta-adrenergic receptor: the single polypeptide confers catecholamine responsiveness to adenylate cyclase.Nature 306, no. 5943 (December 8, 1983): 562–66. https://doi.org/10.1038/306562a0.
Cerione RA, Strulovici B, Benovic JL, Lefkowitz RJ, Caron MG. Pure beta-adrenergic receptor: the single polypeptide confers catecholamine responsiveness to adenylate cyclase. Nature. 1983 Dec 8;306(5943):562–6.
Cerione, R. A., et al. “Pure beta-adrenergic receptor: the single polypeptide confers catecholamine responsiveness to adenylate cyclase.Nature, vol. 306, no. 5943, Dec. 1983, pp. 562–66. Pubmed, doi:10.1038/306562a0.
Cerione RA, Strulovici B, Benovic JL, Lefkowitz RJ, Caron MG. Pure beta-adrenergic receptor: the single polypeptide confers catecholamine responsiveness to adenylate cyclase. Nature. 1983 Dec 8;306(5943):562–566.
Journal cover image

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

December 8, 1983

Volume

306

Issue

5943

Start / End Page

562 / 566

Location

England

Related Subject Headings

  • Xenopus laevis
  • Receptors, Adrenergic, beta
  • Membranes, Artificial
  • Lung
  • Isoproterenol
  • Guinea Pigs
  • General Science & Technology
  • Erythrocytes
  • Dose-Response Relationship, Drug
  • Cricetinae