Cloning and expression of a human kidney cDNA for an alpha 2-adrenergic receptor subtype.
Journal Article (Journal Article)
An alpha 2-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet alpha 2-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet alpha 2-adrenergic receptor and is consistent with the structure of other members of the family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the alpha 2-adrenergic ligand [3H]rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the alpha 2B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet alpha 2-adrenergic receptor (alpha 2A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help in developing more selective alpha-adrenergic ligands.
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Duke Authors
Cited Authors
- Regan, JW; Kobilka, TS; Yang-Feng, TL; Caron, MG; Lefkowitz, RJ; Kobilka, BK
Published Date
- September 1988
Published In
Volume / Issue
- 85 / 17
Start / End Page
- 6301 - 6305
PubMed ID
- 2842764
Pubmed Central ID
- PMC281957
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.85.17.6301
Language
- eng
Conference Location
- United States