Cloning and expression of a human kidney cDNA for an alpha 2-adrenergic receptor subtype.

Journal Article

An alpha 2-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet alpha 2-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet alpha 2-adrenergic receptor and is consistent with the structure of other members of the family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the alpha 2-adrenergic ligand [3H]rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the alpha 2B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet alpha 2-adrenergic receptor (alpha 2A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help in developing more selective alpha-adrenergic ligands.

Full Text

Duke Authors

Cited Authors

  • Regan, JW; Kobilka, TS; Yang-Feng, TL; Caron, MG; Lefkowitz, RJ; Kobilka, BK

Published Date

  • September 1988

Published In

Volume / Issue

  • 85 / 17

Start / End Page

  • 6301 - 6305

PubMed ID

  • 2842764

International Standard Serial Number (ISSN)

  • 0027-8424

Language

  • eng

Conference Location

  • United States