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The human placenta--a rich source of beta-adrenergic receptors: characterization of the receptors in particulate and solubilized preparations.

Publication ,  Journal Article
Schocken, DD; Caron, MG; Lefkowitz, RJ
Published in: J Clin Endocrinol Metab
June 1980

A crude particulate fraction of human placenta possesses a high concentration of beta-adrenergic receptors, as determined by (-)-[3H]dihydroalprenolol binding (approximately 240 fmol/mg protein; Kd approximately 2 nM). The sites display all the typical characteristics of beta-adrenergic receptors, including rapid and reversible kinetics, saturability, and appropriate specificity and stereospecificity. Computer modelling of ligand binding data indicate that the binding of (-)-[3H]dihydroalprenolol to these sites conforms closely to the pattern anticipated for interactions of the ligand with a homogeneous class of receptors according to the law of mass action. The rejeptors are readily solubilized with digitonin, retaining their typical beta-adrenergic characteristics. The human placenta is likely to be a particularly useful source of beta-adrenergic receptors for purification because of its high receptor content and its ready availability in substantial quantities.

Duke Scholars

Published In

J Clin Endocrinol Metab

DOI

ISSN

0021-972X

Publication Date

June 1980

Volume

50

Issue

6

Start / End Page

1082 / 1088

Location

United States

Related Subject Headings

  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic
  • Pregnancy
  • Placenta
  • Kinetics
  • Humans
  • Female
  • Endocrinology & Metabolism
  • Dihydroalprenolol
  • Binding, Competitive
 

Citation

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Schocken, D. D., Caron, M. G., & Lefkowitz, R. J. (1980). The human placenta--a rich source of beta-adrenergic receptors: characterization of the receptors in particulate and solubilized preparations. J Clin Endocrinol Metab, 50(6), 1082–1088. https://doi.org/10.1210/jcem-50-6-1082
Schocken, D. D., M. G. Caron, and R. J. Lefkowitz. “The human placenta--a rich source of beta-adrenergic receptors: characterization of the receptors in particulate and solubilized preparations.J Clin Endocrinol Metab 50, no. 6 (June 1980): 1082–88. https://doi.org/10.1210/jcem-50-6-1082.
Schocken, D. D., et al. “The human placenta--a rich source of beta-adrenergic receptors: characterization of the receptors in particulate and solubilized preparations.J Clin Endocrinol Metab, vol. 50, no. 6, June 1980, pp. 1082–88. Pubmed, doi:10.1210/jcem-50-6-1082.
Journal cover image

Published In

J Clin Endocrinol Metab

DOI

ISSN

0021-972X

Publication Date

June 1980

Volume

50

Issue

6

Start / End Page

1082 / 1088

Location

United States

Related Subject Headings

  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic
  • Pregnancy
  • Placenta
  • Kinetics
  • Humans
  • Female
  • Endocrinology & Metabolism
  • Dihydroalprenolol
  • Binding, Competitive