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Beta-adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids.

Publication ,  Journal Article
Collins, S; Caron, MG; Lefkowitz, RJ
Published in: J Biol Chem
July 5, 1988

Steroid hormones modulate adrenergic receptor responsiveness and receptor number. To investigate the regulation of the beta 2-adrenergic receptor gene by glucocorticoids we examined the effects of the synthetic glucocorticoid agonist triamcinolone acetonide on the expression of beta 2-adrenergic receptors in DDT1MF-2 hamster smooth muscle cells. Glucocorticoid treatment (1 X 10(-7) M) produced a 2.2 +/- 0.4-fold (n = 8) increase in beta 2-adrenergic receptor number (maximum) between 6 and 12 h) as determined by radioligand binding and a similar increase in catecholamine-stimulated adenylate cyclase activity. Steady-state levels of beta 2-adrenergic receptor mRNA, analyzed by Northern blot hybridization, were increased 2.4 +/- 0.4-fold (n = 6) within 1 h, while actin mRNA levels were unchanged throughout the experiment. These steroid-induced increases in beta 2-adrenergic receptor mRNA returned to control levels by 24 h and were followed by a much slower decline in beta 2-adrenergic receptor in plasma membranes. The rate of beta 2-adrenergic receptor gene transcription, assessed by nuclear run-off transcription assays, increased 3.1 +/- 0.1-fold (n = 2) in cells treated for 30 min with 1 X 10(-7) M triamcinolone acetonide. These studies indicate that glucocorticoids regulate the beta 2-adrenergic receptor-adenylate cyclase system by controlling the rate of transcription of the beta 2-adrenergic receptor gene and hence the responsiveness of the enzyme to catecholamine stimulation.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

July 5, 1988

Volume

263

Issue

19

Start / End Page

9067 / 9070

Location

United States

Related Subject Headings

  • Triamcinolone Acetonide
  • Transcription, Genetic
  • Receptors, Adrenergic, beta
  • RNA, Messenger
  • Plasmids
  • Nucleic Acid Hybridization
  • Muscle, Smooth
  • Genes
  • Gene Expression Regulation
  • Cricetinae
 

Citation

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Collins, S., Caron, M. G., & Lefkowitz, R. J. (1988). Beta-adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids. J Biol Chem, 263(19), 9067–9070.
Collins, S., M. G. Caron, and R. J. Lefkowitz. “Beta-adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids.J Biol Chem 263, no. 19 (July 5, 1988): 9067–70.
Collins S, Caron MG, Lefkowitz RJ. Beta-adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids. J Biol Chem. 1988 Jul 5;263(19):9067–70.
Collins, S., et al. “Beta-adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids.J Biol Chem, vol. 263, no. 19, July 1988, pp. 9067–70.
Collins S, Caron MG, Lefkowitz RJ. Beta-adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids. J Biol Chem. 1988 Jul 5;263(19):9067–9070.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

July 5, 1988

Volume

263

Issue

19

Start / End Page

9067 / 9070

Location

United States

Related Subject Headings

  • Triamcinolone Acetonide
  • Transcription, Genetic
  • Receptors, Adrenergic, beta
  • RNA, Messenger
  • Plasmids
  • Nucleic Acid Hybridization
  • Muscle, Smooth
  • Genes
  • Gene Expression Regulation
  • Cricetinae