Functional interaction between monoamine plasma membrane transporters and the synaptic PDZ domain-containing protein PICK1.


Journal Article

PDZ domain-containing proteins play an important role in the targeting and localization of synaptic membrane proteins. Here, we report an interaction between the PDZ domain-containing protein PICK1 and monoamine neurotransmitter transporters in vitro and in vivo. In dopaminergic neurons, PICK1 colocalizes with the dopamine transporter (DAT) and forms a stable protein complex. Coexpression of PICK1 with DAT in mammalian cells and neurons in culture results in colocalization of the two proteins in a cluster pattern and an enhancement of DAT uptake activity through an increase in the number of plasma membrane DAT. Deletion of the PDZ binding site at the carboxyl terminus of DAT abolishes its association with PICK1 and impairs the localization of the transporter in neurons. These findings indicate a role for PDZ-mediated protein interactions in the localization, expression, and function of monoamine transporters.

Full Text

Duke Authors

Cited Authors

  • Torres, GE; Yao, WD; Mohn, AR; Quan, H; Kim, KM; Levey, AI; Staudinger, J; Caron, MG

Published Date

  • April 2001

Published In

Volume / Issue

  • 30 / 1

Start / End Page

  • 121 - 134

PubMed ID

  • 11343649

Pubmed Central ID

  • 11343649

International Standard Serial Number (ISSN)

  • 0896-6273

Digital Object Identifier (DOI)

  • 10.1016/s0896-6273(01)00267-7


  • eng

Conference Location

  • United States