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Ouabain-sensitive Na+-K+ ATPase pumps in cultured human retinal pigment epithelium.

Publication ,  Journal Article
Jaffe, GJ; Burke, JM; Geroski, DH
Published in: Exp Eye Res
January 1989

The expression of Na+-K+ ATPase pumps was studied in cultured human retinal pigment epithelium (RPE). Pump site number was measured by quantitation of the specific binding of [3H]ouabain to cultures of varying density. Specific binding of [3H]ouabain was time- and concentration-dependent, and was inhibited by potassium and by excess unlabeled ouabain. Estimates of pump site number based upon specific [3H]ouabain binding indicated that the number of pumps per RPE cell was maximal in sparse cultures and declined six-fold as cultures became confluent. Pump activity, determined by measurement of specific 86Rb (rubidium) uptake, was also greater in sparse than in dense cultures. Quantitation of [3H]thymidine incorporation as a measure of cell proliferation demonstrated that proliferation in RPE cultures decreased logarithmically as culture density increased. Increased pump site number in cultured RPE, therefore, correlated with increased cell proliferation and decreased culture density. We conclude that human RPE express ouabain-sensitive Na+-K+ ATPase in vitro and maximal expression is observed in sparse, proliferating cultures.

Duke Scholars

Published In

Exp Eye Res

DOI

ISSN

0014-4835

Publication Date

January 1989

Volume

48

Issue

1

Start / End Page

61 / 68

Location

England

Related Subject Headings

  • Thymidine
  • Sodium-Potassium-Exchanging ATPase
  • Sodium
  • Potassium
  • Pigment Epithelium of Eye
  • Ouabain
  • Ophthalmology & Optometry
  • Mitosis
  • Humans
  • Dose-Response Relationship, Drug
 

Citation

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ICMJE
MLA
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Jaffe, G. J., Burke, J. M., & Geroski, D. H. (1989). Ouabain-sensitive Na+-K+ ATPase pumps in cultured human retinal pigment epithelium. Exp Eye Res, 48(1), 61–68. https://doi.org/10.1016/0014-4835(89)90019-5
Jaffe, G. J., J. M. Burke, and D. H. Geroski. “Ouabain-sensitive Na+-K+ ATPase pumps in cultured human retinal pigment epithelium.Exp Eye Res 48, no. 1 (January 1989): 61–68. https://doi.org/10.1016/0014-4835(89)90019-5.
Jaffe GJ, Burke JM, Geroski DH. Ouabain-sensitive Na+-K+ ATPase pumps in cultured human retinal pigment epithelium. Exp Eye Res. 1989 Jan;48(1):61–8.
Jaffe, G. J., et al. “Ouabain-sensitive Na+-K+ ATPase pumps in cultured human retinal pigment epithelium.Exp Eye Res, vol. 48, no. 1, Jan. 1989, pp. 61–68. Pubmed, doi:10.1016/0014-4835(89)90019-5.
Jaffe GJ, Burke JM, Geroski DH. Ouabain-sensitive Na+-K+ ATPase pumps in cultured human retinal pigment epithelium. Exp Eye Res. 1989 Jan;48(1):61–68.
Journal cover image

Published In

Exp Eye Res

DOI

ISSN

0014-4835

Publication Date

January 1989

Volume

48

Issue

1

Start / End Page

61 / 68

Location

England

Related Subject Headings

  • Thymidine
  • Sodium-Potassium-Exchanging ATPase
  • Sodium
  • Potassium
  • Pigment Epithelium of Eye
  • Ouabain
  • Ophthalmology & Optometry
  • Mitosis
  • Humans
  • Dose-Response Relationship, Drug