Hypoxia precedes the development of experimental preretinal neovascularization.
Journal Article
Background
Although the mechanism of preretinal neovascular growth in the cell-injected rabbit eye model is not known, it has been proposed that the initial vasodilation and eventual development of neovascularization may be attributable to inflammatory mediators. However, an alternative explanation involving hypoxia has not been considered. The purpose of this study was to measure preretinal oxygen tension prior to the development of preretinal neovascularization in the cell-injected rabbit eye.Methods
In the rabbit, intravitreous injections of 250,000 homologous dermal fibroblasts were performed on one eye; the fellow (control) eye was injected with vehicle. Preretinal oxygen tension over the myelin wing was measured using 19F-NMR spectroscopy of a 30-microliters droplet of perfluorocarbon previously injected into the preretinal vitreous.Results
Compared to control eyes, fibroblast-injected eyes showed a 1.7-fold decrease in preretinal oxygen tension from the first time studied (1 day after cell injection) through the development of visible neovascularization. Hypoxia occurred without coexisting ophthalmoscopic evidence of vascular occlusion or, on days 1 and 3 after cell injection, retinal detachment.Conclusion
This result demonstrates for the first time that preretinal hypoxia precedes the development of preretinal neovascularization in the fibroblast-injected rabbit eye.Full Text
Duke Authors
Cited Authors
- Handa, JT; Berkowitz, BA; Wilson, CA; Ando, N; Sen, HA; Jaffe, GJ
Published Date
- January 1996
Published In
Volume / Issue
- 234 / 1
Start / End Page
- 43 - 46
PubMed ID
- 8750849
Pubmed Central ID
- 8750849
Electronic International Standard Serial Number (EISSN)
- 1435-702X
International Standard Serial Number (ISSN)
- 0721-832X
Digital Object Identifier (DOI)
- 10.1007/bf00186517
Language
- eng