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Retinal pigment epithelial cells of the posterior pole have fewer Na/K adenosine triphosphatase pumps than peripheral cells.

Publication ,  Journal Article
Burke, JM; McKay, BS; Jaffe, GJ
Published in: Invest Ophthalmol Vis Sci
June 1991

The density of Na/K adenosine triphosphatase (ATPase) pumps in retinal pigment epithelial (RPE) cells in different retinal regions was quantified by measuring the binding of 3H-ouabain to RPE in cow and human eyecups. In bovine eyes, pump density was estimated in RPE samples isolated from three retinal regions outlined with a 7-mm trephine: one from the posterior pole in the area centralis and two from the superior, equatorial retina representing unpigmented (in the tapetum) and pigmented zones. In human eyes, RPE samples were isolated from a posterior region centered around the macula and one superior region. Ouabain binding to RPE of the posterior pole of both species was approximately 40-60% lower than binding to RPE of more peripheral regions in the same eyes. For bovine eyes, ouabain binding did not differ between pigmented and unpigmented cells of the superior retina, suggesting that reduced binding in the relatively amelanotic posterior cells was not related to levels of pigmentation. For human RPE, binding to posterior cells was lower in eyes from donors of all ages (range, 17-90 yr). The data suggest that Na/K ATPase pump site density is lower in posterior RPE cells of both bovine and human eyes, perhaps due to a regional difference in requirements for ionic regulation.

Duke Scholars

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

June 1991

Volume

32

Issue

7

Start / End Page

2042 / 2046

Location

United States

Related Subject Headings

  • Sodium-Potassium-Exchanging ATPase
  • Random Allocation
  • Pigment Epithelium of Eye
  • Ouabain
  • Ophthalmology & Optometry
  • Middle Aged
  • Macula Lutea
  • Humans
  • Fovea Centralis
  • Cattle
 

Citation

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MLA
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Burke, J. M., McKay, B. S., & Jaffe, G. J. (1991). Retinal pigment epithelial cells of the posterior pole have fewer Na/K adenosine triphosphatase pumps than peripheral cells. Invest Ophthalmol Vis Sci, 32(7), 2042–2046.
Burke, J. M., B. S. McKay, and G. J. Jaffe. “Retinal pigment epithelial cells of the posterior pole have fewer Na/K adenosine triphosphatase pumps than peripheral cells.Invest Ophthalmol Vis Sci 32, no. 7 (June 1991): 2042–46.
Burke, J. M., et al. “Retinal pigment epithelial cells of the posterior pole have fewer Na/K adenosine triphosphatase pumps than peripheral cells.Invest Ophthalmol Vis Sci, vol. 32, no. 7, June 1991, pp. 2042–46.
Burke JM, McKay BS, Jaffe GJ. Retinal pigment epithelial cells of the posterior pole have fewer Na/K adenosine triphosphatase pumps than peripheral cells. Invest Ophthalmol Vis Sci. 1991 Jun;32(7):2042–2046.

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

June 1991

Volume

32

Issue

7

Start / End Page

2042 / 2046

Location

United States

Related Subject Headings

  • Sodium-Potassium-Exchanging ATPase
  • Random Allocation
  • Pigment Epithelium of Eye
  • Ouabain
  • Ophthalmology & Optometry
  • Middle Aged
  • Macula Lutea
  • Humans
  • Fovea Centralis
  • Cattle