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Aquaporin-1 channels in human retinal pigment epithelium: role in transepithelial water movement.

Publication ,  Journal Article
Stamer, WD; Bok, D; Hu, J; Jaffe, GJ; McKay, BS
Published in: Invest Ophthalmol Vis Sci
June 2003

PURPOSE: Aquaporin (AQP) is a hexahelical integral membrane protein that functions as a constitutive channel for water and regulated channel for cations in fluid transporting tissues, including many in the eye. Although AQP1 has been cloned from a cDNA library prepared from cultures of retinal pigment epithelial (RPE) cells isolated from human fetal tissue, three separate studies failed with various immunochemical techniques to detect AQP1 protein in adult human or rat RPE preparations. The purpose of this study was to examine specifically the expression and distribution of AQP1 in adult human RPE in situ by using alternative methodologies and model systems and to determine the contribution of AQP1 to water movement across cultured RPE cells isolated from human cadaveric and fetal eyes. METHODS: AQP1 in human RPE in situ was determined after biotinylation of proteins on cell surfaces and streptavidin chromatography, followed by immunoblot analyses. AQP1 distribution in a polarized in vitro RPE model was determined with indirect immunofluorescence confocal microscopy. The role of channel-mediated transport of water across RPE cell monolayers on filters was assessed by osmotic challenge assay. Expression levels of AQP1 were controlled with an adenovirus expression system and monitored by immunoblot analyses. RESULTS: AQP1 protein was detected in human RPE in situ and in cultures of human adult and fetal RPE cells. In functional assays, AQP1 facilitated water movement across RPE monolayers in an expression-dependent manner in two complementary model systems. CONCLUSION: The expression of AQP1 by RPE in vivo probably contributes to the efficient transepithelial water transport across RPE, maintains retinal attachment, and prevents subretinal edema.

Duke Scholars

Published In

Invest Ophthalmol Vis Sci

DOI

ISSN

0146-0404

Publication Date

June 2003

Volume

44

Issue

6

Start / End Page

2803 / 2808

Location

United States

Related Subject Headings

  • Water
  • Pigment Epithelium of Eye
  • Ophthalmology & Optometry
  • Middle Aged
  • Microscopy, Confocal
  • Humans
  • Genetic Vectors
  • Gene Expression Regulation
  • Fluorescent Antibody Technique, Indirect
  • Fetus
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Stamer, W. D., Bok, D., Hu, J., Jaffe, G. J., & McKay, B. S. (2003). Aquaporin-1 channels in human retinal pigment epithelium: role in transepithelial water movement. Invest Ophthalmol Vis Sci, 44(6), 2803–2808. https://doi.org/10.1167/iovs.03-0001
Stamer, W Daniel, Dean Bok, Jane Hu, Glenn J. Jaffe, and Brian S. McKay. “Aquaporin-1 channels in human retinal pigment epithelium: role in transepithelial water movement.Invest Ophthalmol Vis Sci 44, no. 6 (June 2003): 2803–8. https://doi.org/10.1167/iovs.03-0001.
Stamer WD, Bok D, Hu J, Jaffe GJ, McKay BS. Aquaporin-1 channels in human retinal pigment epithelium: role in transepithelial water movement. Invest Ophthalmol Vis Sci. 2003 Jun;44(6):2803–8.
Stamer, W. Daniel, et al. “Aquaporin-1 channels in human retinal pigment epithelium: role in transepithelial water movement.Invest Ophthalmol Vis Sci, vol. 44, no. 6, June 2003, pp. 2803–08. Pubmed, doi:10.1167/iovs.03-0001.
Stamer WD, Bok D, Hu J, Jaffe GJ, McKay BS. Aquaporin-1 channels in human retinal pigment epithelium: role in transepithelial water movement. Invest Ophthalmol Vis Sci. 2003 Jun;44(6):2803–2808.

Published In

Invest Ophthalmol Vis Sci

DOI

ISSN

0146-0404

Publication Date

June 2003

Volume

44

Issue

6

Start / End Page

2803 / 2808

Location

United States

Related Subject Headings

  • Water
  • Pigment Epithelium of Eye
  • Ophthalmology & Optometry
  • Middle Aged
  • Microscopy, Confocal
  • Humans
  • Genetic Vectors
  • Gene Expression Regulation
  • Fluorescent Antibody Technique, Indirect
  • Fetus