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Activin expression by cultured human retinal pigment epithelial cells.

Publication ,  Journal Article
Jaffe, GJ; Harrison, CE; Lui, GM; Roberts, WL; Goldsmith, PC; Mesiano, S; Jaffe, RB
Published in: Invest Ophthalmol Vis Sci
June 1994

PURPOSE: To determine whether human retinal pigment epithelial (hRPE) cells produce activin, a growth factor in the transforming growth factor beta family, and to characterize growth regulatory effects of activin on retinal pigment epithelium. METHODS: mRNA expression was examined using polymerase chain reaction with primers specific for the beta A and beta B chains of activin and by slot blot analysis with a probe specific for the beta A chain. Protein localization was determined immunocytochemically using antibodies specific for the beta A chain of activin and intact activin A. The effect of activin A on DNA synthesis was studied by measuring (3H) thymidine incorporation after cells were exposed to recombinant human activin A (rhA). Growth regulatory effects of rhA on hRPE cells were examined with cell growth assays. RESULTS: beta A mRNA was expressed constitutively in 8/8 cells lines tested. beta B mRNA was not expressed in any of the six cell lines tested but was expressed in human ovarian granulosa cell controls. Positive immunostaining was observed for both the beta A chain and intact activin A. (3H) thymidine incorporation was inhibited 44% (P < 0.025), 45% (P < 0.025), and 44% (P < 0.015) when RPE cells were exposed to 100 ng/ml rhA and grown in serum-free medium, medium with 0.5% serum, and 1% serum, respectively. Cell growth was inhibited 33.2% (P = 0.0001) after RPE cells were exposed to 100 ng/ml rhA for 8 days. CONCLUSIONS: These results suggest that activin A can act as an autocrine-paracrine growth regulator in RPE cells and may help control cellular growth in ocular development and proliferative eye disease.

Duke Scholars

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

June 1994

Volume

35

Issue

7

Start / End Page

2924 / 2931

Location

United States

Related Subject Headings

  • RNA, Messenger
  • Polymerase Chain Reaction
  • Pigment Epithelium of Eye
  • Ophthalmology & Optometry
  • Inhibins
  • Immunoblotting
  • Humans
  • Growth Substances
  • DNA
  • Cells, Cultured
 

Citation

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Jaffe, G. J., Harrison, C. E., Lui, G. M., Roberts, W. L., Goldsmith, P. C., Mesiano, S., & Jaffe, R. B. (1994). Activin expression by cultured human retinal pigment epithelial cells. Invest Ophthalmol Vis Sci, 35(7), 2924–2931.
Jaffe, G. J., C. E. Harrison, G. M. Lui, W. L. Roberts, P. C. Goldsmith, S. Mesiano, and R. B. Jaffe. “Activin expression by cultured human retinal pigment epithelial cells.Invest Ophthalmol Vis Sci 35, no. 7 (June 1994): 2924–31.
Jaffe GJ, Harrison CE, Lui GM, Roberts WL, Goldsmith PC, Mesiano S, et al. Activin expression by cultured human retinal pigment epithelial cells. Invest Ophthalmol Vis Sci. 1994 Jun;35(7):2924–31.
Jaffe, G. J., et al. “Activin expression by cultured human retinal pigment epithelial cells.Invest Ophthalmol Vis Sci, vol. 35, no. 7, June 1994, pp. 2924–31.
Jaffe GJ, Harrison CE, Lui GM, Roberts WL, Goldsmith PC, Mesiano S, Jaffe RB. Activin expression by cultured human retinal pigment epithelial cells. Invest Ophthalmol Vis Sci. 1994 Jun;35(7):2924–2931.

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

June 1994

Volume

35

Issue

7

Start / End Page

2924 / 2931

Location

United States

Related Subject Headings

  • RNA, Messenger
  • Polymerase Chain Reaction
  • Pigment Epithelium of Eye
  • Ophthalmology & Optometry
  • Inhibins
  • Immunoblotting
  • Humans
  • Growth Substances
  • DNA
  • Cells, Cultured