Skip to main content
Journal cover image

Binding, coupling, and mRNA subtype heterogeneity of alpha 1-adrenergic receptors in cultured human RPE.

Publication ,  Journal Article
Moroi-Fetters, SE; Earley, O; Hirakata, A; Caron, MG; Jaffe, GJ
Published in: Exp Eye Res
May 1995

In retinal pigment epithelium, apically applied epinephrine changes the conductance of specific ions which subsequently affects the membrane voltage and stimulates transepithelial fluid transport. In this investigation, myo-[3H]inositol radiotracer studies, radioligand binding with [125I]HEAT, and ribonuclease protection assays were performed to examine the coupling of this receptor to phosphoinositide hydrolysis and the specific mRNA subtypes expressed in cultured human retinal pigment epithelial cells. After labeling second to sixth passage cells with 3-muCi myo-[3H]inositol for 24 hr, epinephrine caused a dose- and time-dependent increase in [3H]inositol phosphate products (EC50 of 0.7 microM). This stimulation was antagonized by prazosin but not by propranolol. The effect of epinephrine was potentiated by the presence of the monoamine oxidase inhibitor, pargyline (10 microM). Pertussis toxin (1 microgram per well) attenuated the stimulatory effect of epinephrine. In the radioligand binding assays, [125I]HEAT binding sites varied among different cell lines, with a range of 44 to 200 fmol (mg protein)-1. Using a ribonuclease protection assay, alpha 1D and alpha 1B, but not alpha 1C, adrenergic mRNA subtypes were detected in cultured human cells. Collectively, these results show that the catecholamines act on a potentially heterogeneous population of alpha 1-adrenergic receptors coupled to phospholipase C by a pertussis toxin-sensitive G protein.

Duke Scholars

Published In

Exp Eye Res

DOI

ISSN

0014-4835

Publication Date

May 1995

Volume

60

Issue

5

Start / End Page

527 / 532

Location

England

Related Subject Headings

  • Virulence Factors, Bordetella
  • Type C Phospholipases
  • Stimulation, Chemical
  • Receptors, Adrenergic, alpha-1
  • RNA, Messenger
  • Protein Binding
  • Propranolol
  • Prazosin
  • Pigment Epithelium of Eye
  • Phosphatidylinositols
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Moroi-Fetters, S. E., Earley, O., Hirakata, A., Caron, M. G., & Jaffe, G. J. (1995). Binding, coupling, and mRNA subtype heterogeneity of alpha 1-adrenergic receptors in cultured human RPE. Exp Eye Res, 60(5), 527–532. https://doi.org/10.1016/s0014-4835(05)80067-3
Moroi-Fetters, S. E., O. Earley, A. Hirakata, M. G. Caron, and G. J. Jaffe. “Binding, coupling, and mRNA subtype heterogeneity of alpha 1-adrenergic receptors in cultured human RPE.Exp Eye Res 60, no. 5 (May 1995): 527–32. https://doi.org/10.1016/s0014-4835(05)80067-3.
Moroi-Fetters SE, Earley O, Hirakata A, Caron MG, Jaffe GJ. Binding, coupling, and mRNA subtype heterogeneity of alpha 1-adrenergic receptors in cultured human RPE. Exp Eye Res. 1995 May;60(5):527–32.
Moroi-Fetters, S. E., et al. “Binding, coupling, and mRNA subtype heterogeneity of alpha 1-adrenergic receptors in cultured human RPE.Exp Eye Res, vol. 60, no. 5, May 1995, pp. 527–32. Pubmed, doi:10.1016/s0014-4835(05)80067-3.
Moroi-Fetters SE, Earley O, Hirakata A, Caron MG, Jaffe GJ. Binding, coupling, and mRNA subtype heterogeneity of alpha 1-adrenergic receptors in cultured human RPE. Exp Eye Res. 1995 May;60(5):527–532.
Journal cover image

Published In

Exp Eye Res

DOI

ISSN

0014-4835

Publication Date

May 1995

Volume

60

Issue

5

Start / End Page

527 / 532

Location

England

Related Subject Headings

  • Virulence Factors, Bordetella
  • Type C Phospholipases
  • Stimulation, Chemical
  • Receptors, Adrenergic, alpha-1
  • RNA, Messenger
  • Protein Binding
  • Propranolol
  • Prazosin
  • Pigment Epithelium of Eye
  • Phosphatidylinositols