cDNA cloning of an intracellular form of the human interleukin 1 receptor antagonist associated with epithelium.

Journal Article

A cDNA encoding a receptor antagonist of interleukin 1 (IL-1ra), secreted from human monocytes, has recently been isolated and sequenced [Eisenberg, S. P., Evans, R. J., Arend, W. P., Verderber, E., Brewer, M. T., Hannum, C. H. & Thompson, R. C. (1990) Nature (London) 343, 341-346]. We have identified another version of this IL-1ra, which is predominantly expressed in epithelial cells. This IL-1ra lacks a leader sequence and, thus, is probably intracellular. Both proteins are derived from the same gene through use of an alternative transcriptional start site and internal splice-acceptor site. Expression of intracellular IL-1ra cDNA in COS cells demonstrated that the intracellular product specifically inhibited exogenous interleukin 1-dependent responses. Keratinocytes were shown to contain significant amounts of nonsecreted IL-1ra protein. Constitutive expression of the intracellular IL-1ra may be an intracellular defensive mechanism in exposed epithelial cells and/or may serve to regulate autocrine interleukin 1-mediated pathways of differentiation.

Full Text

Duke Authors

Cited Authors

  • Haskill, S; Martin, G; Van Le, L; Morris, J; Peace, A; Bigler, CF; Jaffe, GJ; Hammerberg, C; Sporn, SA; Fong, S

Published Date

  • May 1, 1991

Published In

Volume / Issue

  • 88 / 9

Start / End Page

  • 3681 - 3685

PubMed ID

  • 1827201

International Standard Serial Number (ISSN)

  • 0027-8424

Language

  • eng

Conference Location

  • United States