cDNA cloning of an intracellular form of the human interleukin 1 receptor antagonist associated with epithelium.
Journal Article (Journal Article)
A cDNA encoding a receptor antagonist of interleukin 1 (IL-1ra), secreted from human monocytes, has recently been isolated and sequenced [Eisenberg, S. P., Evans, R. J., Arend, W. P., Verderber, E., Brewer, M. T., Hannum, C. H. & Thompson, R. C. (1990) Nature (London) 343, 341-346]. We have identified another version of this IL-1ra, which is predominantly expressed in epithelial cells. This IL-1ra lacks a leader sequence and, thus, is probably intracellular. Both proteins are derived from the same gene through use of an alternative transcriptional start site and internal splice-acceptor site. Expression of intracellular IL-1ra cDNA in COS cells demonstrated that the intracellular product specifically inhibited exogenous interleukin 1-dependent responses. Keratinocytes were shown to contain significant amounts of nonsecreted IL-1ra protein. Constitutive expression of the intracellular IL-1ra may be an intracellular defensive mechanism in exposed epithelial cells and/or may serve to regulate autocrine interleukin 1-mediated pathways of differentiation.
Full Text
Duke Authors
Cited Authors
- Haskill, S; Martin, G; Van Le, L; Morris, J; Peace, A; Bigler, CF; Jaffe, GJ; Hammerberg, C; Sporn, SA; Fong, S
Published Date
- May 1, 1991
Published In
Volume / Issue
- 88 / 9
Start / End Page
- 3681 - 3685
PubMed ID
- 1827201
Pubmed Central ID
- PMC51516
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.88.9.3681
Language
- eng
Conference Location
- United States