Organization of the human zeta-crystallin/quinone reductase gene (CRYZ).

Journal Article (Journal Article)

zeta-Crystallin is a protein highly expressed in the lens of guinea pigs and camels, where it comprises about 10% of the total soluble protein. It has recently been characterized as a novel quinone oxidoreductase present in a variety of mammalian tissues. We report here the isolation and characterization of the human zeta-crystallin gene (CRYZ) and its processed pseudogene. The functional gene is composed of nine exons and spans about 20 kb. The 5'-flanking region of the gene is rich in G and C (58%) and lacks TATA and CAAT boxes. Previous analysis of the guinea pig gene revealed the presence of two different promoters, one responsible for the high lens-specific expression and the other for expression at the enzymatic level in numerous tissues. Comparative analysis with the guinea pig gene shows that a region of approximately 2.5 kb that includes the promoter responsible for the high expression in the lens in guinea pig is not present in the human gene.

Full Text

Duke Authors

Cited Authors

  • Gonzalez, P; Rao, PV; Zigler, JS

Published Date

  • May 15, 1994

Published In

Volume / Issue

  • 21 / 2

Start / End Page

  • 317 - 324

PubMed ID

  • 8088825

International Standard Serial Number (ISSN)

  • 0888-7543

Digital Object Identifier (DOI)

  • 10.1006/geno.1994.1272


  • eng

Conference Location

  • United States