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CD11c(+)B220(+)Gr-1(+) cells in mouse lymph nodes and spleen display characteristics of plasmacytoid dendritic cells.

Publication ,  Journal Article
Nakano, H; Yanagita, M; Gunn, MD
Published in: J Exp Med
October 15, 2001

Human plasmacytoid dendritic cells (pDCs) are major producers of IFNalpha, are activated by CpG motifs, and are believed to enter lymph nodes (LNs) via L-selectin dependent extravasation across high endothelial venules. To identify a similar murine DC type, CD11c(+) cells in the LNs of L-selectin-deficient and control BALB/c mice were compared, revealing a population of CD11c(+)CD11b(-) cells that is reduced 85% in the LNs of L-selectin-deficient mice. These cells are Gr-1(+)B220(+)CD19(-), either CD4(+) or CD8(+), and localize within T cell zones of LNs. Freshly isolated CD11c(+)Gr-1(+) cells express major histocompatibility complex class II at low levels, display a plasmacytoid morphology, and survive poorly in culture. Their survival is increased and they develop a DC-like morphology in interleukin 3 and CpG. Like human pDCs, CD11c(+)Gr-1(+) cells stimulate T cell proliferation after activation with CpG and produce IFNalpha after stimulation with influenza virus. These cells also display a strain-specific variation in frequency, being fivefold increased in the LNs of BALB/c relative to C57BL/6 mice. These CD11c(+)CD11b(-)B220(+)Gr-1(+) cells appear to be the murine equivalent of human pDCs.

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Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

October 15, 2001

Volume

194

Issue

8

Start / End Page

1171 / 1178

Location

United States

Related Subject Headings

  • Spleen
  • Plasma Cells
  • Orthomyxoviridae
  • Mice, Nude
  • Mice, Knockout
  • Mice, Inbred DBA
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Lymph Nodes
 

Citation

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Nakano, H., Yanagita, M., & Gunn, M. D. (2001). CD11c(+)B220(+)Gr-1(+) cells in mouse lymph nodes and spleen display characteristics of plasmacytoid dendritic cells. J Exp Med, 194(8), 1171–1178. https://doi.org/10.1084/jem.194.8.1171
Nakano, H., M. Yanagita, and M. D. Gunn. “CD11c(+)B220(+)Gr-1(+) cells in mouse lymph nodes and spleen display characteristics of plasmacytoid dendritic cells.J Exp Med 194, no. 8 (October 15, 2001): 1171–78. https://doi.org/10.1084/jem.194.8.1171.
Nakano, H., et al. “CD11c(+)B220(+)Gr-1(+) cells in mouse lymph nodes and spleen display characteristics of plasmacytoid dendritic cells.J Exp Med, vol. 194, no. 8, Oct. 2001, pp. 1171–78. Pubmed, doi:10.1084/jem.194.8.1171.

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

October 15, 2001

Volume

194

Issue

8

Start / End Page

1171 / 1178

Location

United States

Related Subject Headings

  • Spleen
  • Plasma Cells
  • Orthomyxoviridae
  • Mice, Nude
  • Mice, Knockout
  • Mice, Inbred DBA
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Lymph Nodes