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Gene duplications at the chemokine locus on mouse chromosome 4: multiple strain-specific haplotypes and the deletion of secondary lymphoid-organ chemokine and EBI-1 ligand chemokine genes in the plt mutation.

Publication ,  Journal Article
Nakano, H; Gunn, MD
Published in: J Immunol
January 1, 2001

The murine paucity of lymph node T cell (plt) mutation leads to abnormalities in leukocyte migration and immune response. The causative defect is thought to be a loss of secondary lymphoid-organ chemokine (SLC) expression in lymphoid tissues. We now find that the plt defect is due to the loss of both SLC and EBI-1 ligand chemokine (ELC) expression in secondary lymphoid organs. In an examination of the plt locus, we find that commonly used inbred mouse strains demonstrate at least three different haplotypes. Polymorphism at this locus is due to duplications of at least four genes, three of them encoding chemokines. At least two cutaneous T cell-attracting chemokine (CTACK), three SLC, and four ELC genes or pseudogenes are present in some haplotypes. All haplotypes share a duplication that includes two SLC genes, which demonstrate different expression patterns, a single functional ELC gene, and an ELC pseudogene. The plt mutation represents a deletion that includes the SLC gene expressed in secondary lymphoid organs and the single functional ELC gene, leaving only an SLC gene that is expressed in lymphatic endothelium and an ELC pseudogene. This lack of CCR7 ligands in the secondary lymphoid organs of plt mice provides a basis for their severe abnormalities in leukocyte migration and immune response.

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Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

January 1, 2001

Volume

166

Issue

1

Start / End Page

361 / 369

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Species Specificity
  • Protein Isoforms
  • Multigene Family
  • Mice, Mutant Strains
  • Mice, Inbred Strains
  • Mice, Inbred DBA
  • Mice, Inbred BALB C
  • Mice
  • Lymph Nodes
 

Citation

APA
Chicago
ICMJE
MLA
NLM

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

January 1, 2001

Volume

166

Issue

1

Start / End Page

361 / 369

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Species Specificity
  • Protein Isoforms
  • Multigene Family
  • Mice, Mutant Strains
  • Mice, Inbred Strains
  • Mice, Inbred DBA
  • Mice, Inbred BALB C
  • Mice
  • Lymph Nodes