Skip to main content

Mice lacking expression of secondary lymphoid organ chemokine have defects in lymphocyte homing and dendritic cell localization.

Publication ,  Journal Article
Gunn, MD; Kyuwa, S; Tam, C; Kakiuchi, T; Matsuzawa, A; Williams, LT; Nakano, H
Published in: J Exp Med
February 1, 1999

Secondary lymphoid organ chemokine (SLC) is expressed in high endothelial venules and in T cell zones of spleen and lymph nodes (LNs) and strongly attracts naive T cells. In mice homozygous for the paucity of lymph node T cell (plt) mutation, naive T cells fail to home to LNs or the lymphoid regions of spleen. Here we demonstrate that expression of SLC is undetectable in plt mice. In addition to the defect in T cell homing, we demonstrate that dendritic cells (DCs) fail to accumulate in spleen and LN T cell zones of plt mice. DC migration to LNs after contact sensitization is also substantially reduced. The physiologic significance of these abnormalities in plt mice is indicated by a markedly increased sensitivity to infection with murine hepatitis virus. The plt mutation maps to the SLC locus; however, the sequence of SLC introns and exons in plt mice is normal. These findings suggest that the abnormalities in plt mice are due to a genetic defect in the expression of SLC and that SLC mediates the entry of naive T cells and antigen-stimulated DCs into the T cell zones of secondary lymphoid organs.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

February 1, 1999

Volume

189

Issue

3

Start / End Page

451 / 460

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Spleen
  • Receptors, Chemokine
  • Receptors, CCR7
  • RNA, Messenger
  • Murine hepatitis virus
  • Mice, Mutant Strains
  • Mice
  • Lymph Nodes
  • Immunology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Gunn, M. D., Kyuwa, S., Tam, C., Kakiuchi, T., Matsuzawa, A., Williams, L. T., & Nakano, H. (1999). Mice lacking expression of secondary lymphoid organ chemokine have defects in lymphocyte homing and dendritic cell localization. J Exp Med, 189(3), 451–460. https://doi.org/10.1084/jem.189.3.451
Gunn, M. D., S. Kyuwa, C. Tam, T. Kakiuchi, A. Matsuzawa, L. T. Williams, and H. Nakano. “Mice lacking expression of secondary lymphoid organ chemokine have defects in lymphocyte homing and dendritic cell localization.J Exp Med 189, no. 3 (February 1, 1999): 451–60. https://doi.org/10.1084/jem.189.3.451.
Gunn MD, Kyuwa S, Tam C, Kakiuchi T, Matsuzawa A, Williams LT, et al. Mice lacking expression of secondary lymphoid organ chemokine have defects in lymphocyte homing and dendritic cell localization. J Exp Med. 1999 Feb 1;189(3):451–60.
Gunn, M. D., et al. “Mice lacking expression of secondary lymphoid organ chemokine have defects in lymphocyte homing and dendritic cell localization.J Exp Med, vol. 189, no. 3, Feb. 1999, pp. 451–60. Pubmed, doi:10.1084/jem.189.3.451.
Gunn MD, Kyuwa S, Tam C, Kakiuchi T, Matsuzawa A, Williams LT, Nakano H. Mice lacking expression of secondary lymphoid organ chemokine have defects in lymphocyte homing and dendritic cell localization. J Exp Med. 1999 Feb 1;189(3):451–460.

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

February 1, 1999

Volume

189

Issue

3

Start / End Page

451 / 460

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Spleen
  • Receptors, Chemokine
  • Receptors, CCR7
  • RNA, Messenger
  • Murine hepatitis virus
  • Mice, Mutant Strains
  • Mice
  • Lymph Nodes
  • Immunology