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RNA subunit of mitochondrial RNA-processing enzyme is induced by contractile activity in striated muscle.

Publication ,  Journal Article
Ordway, GA; Li, K; Hand, GA; Williams, RS
Published in: Am J Physiol
December 1993

A small RNA encoded within the nucleus of yeast and mammalian cells is an essential subunit of a mitochondrial RNA-processing endonuclease (RNase MRP) that generates primers for mitochondrial DNA (mtDNA) replication. We examined expression of MRP-RNA in specialized subtypes of mammalian striated muscles that differ markedly in respiratory activity and in muscles subjected to chronic stimulation via the motor nerve, a potent stimulus to mitochondrial biogenesis. MRP-RNA was more abundant in mitochondria-rich cardiac and slow-twitch skeletal muscles than in glycolytic fast-twitch skeletal muscles. Forced contractile activity resulting from nerve stimulation increased expression of MRP-RNA by 3.5-fold within the first day and by 14-fold within 14 days. Changes in abundance of MRP-RNA preceded but otherwise occurred in parallel to changes in specific activity of citrate synthase, a marker of mitochondrial proliferation shown previously to correlate with mtDNA copy number in this model. Another small RNA (U1) also was induced transiently (1-3 days) by nerve stimulation, but such changes were not sustained and were of less magnitude (< 4-fold) than changes in MRP-RNA. These findings are consistent with the hypothesis that MRP-RNA may have a regulatory function with respect to mtDNA replication and mitochondrial biogenesis.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

December 1993

Volume

265

Issue

6 Pt 1

Start / End Page

C1511 / C1516

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • TATA Box
  • Sequence Deletion
  • Ribonuclease III
  • Rabbits
  • RNA
  • Promoter Regions, Genetic
  • Polymerase Chain Reaction
  • Peroneal Nerve
  • Oligonucleotide Probes
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ordway, G. A., Li, K., Hand, G. A., & Williams, R. S. (1993). RNA subunit of mitochondrial RNA-processing enzyme is induced by contractile activity in striated muscle. Am J Physiol, 265(6 Pt 1), C1511–C1516. https://doi.org/10.1152/ajpcell.1993.265.6.C1511
Ordway, G. A., K. Li, G. A. Hand, and R. S. Williams. “RNA subunit of mitochondrial RNA-processing enzyme is induced by contractile activity in striated muscle.Am J Physiol 265, no. 6 Pt 1 (December 1993): C1511–16. https://doi.org/10.1152/ajpcell.1993.265.6.C1511.
Ordway GA, Li K, Hand GA, Williams RS. RNA subunit of mitochondrial RNA-processing enzyme is induced by contractile activity in striated muscle. Am J Physiol. 1993 Dec;265(6 Pt 1):C1511–6.
Ordway, G. A., et al. “RNA subunit of mitochondrial RNA-processing enzyme is induced by contractile activity in striated muscle.Am J Physiol, vol. 265, no. 6 Pt 1, Dec. 1993, pp. C1511–16. Pubmed, doi:10.1152/ajpcell.1993.265.6.C1511.
Ordway GA, Li K, Hand GA, Williams RS. RNA subunit of mitochondrial RNA-processing enzyme is induced by contractile activity in striated muscle. Am J Physiol. 1993 Dec;265(6 Pt 1):C1511–C1516.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

December 1993

Volume

265

Issue

6 Pt 1

Start / End Page

C1511 / C1516

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • TATA Box
  • Sequence Deletion
  • Ribonuclease III
  • Rabbits
  • RNA
  • Promoter Regions, Genetic
  • Polymerase Chain Reaction
  • Peroneal Nerve
  • Oligonucleotide Probes