Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles.

Published

Journal Article

Foreign genes were expressed in liver and skin cells of live mice by using a new apparatus to accelerate DNA-coated microprojectiles into tissues. After introduction of a plasmid in which the firefly luciferase gene was controlled by the human beta-actin promoter, luciferase activity was detectable for up to 14 days in mouse tissues (skin and liver). In situ hybridization histochemistry revealed that microprojectiles penetrated through multiple cell layers without evidence of tissue injury and that 10-20% of the cells in the bombarded area expressed the foreign gene. An advantage of the new design is that internal organs, such as liver, can be transfected without subjecting the tissue to a vacuum. This procedure potentially is applicable to a wide variety of tissues and cell types for studies of transcriptional control elements and for expression of foreign proteins in intact animals.

Full Text

Duke Authors

Cited Authors

  • Williams, RS; Johnston, SA; Riedy, M; DeVit, MJ; McElligott, SG; Sanford, JC

Published Date

  • April 1, 1991

Published In

Volume / Issue

  • 88 / 7

Start / End Page

  • 2726 - 2730

PubMed ID

  • 2011582

Pubmed Central ID

  • 2011582

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.88.7.2726

Language

  • eng

Conference Location

  • United States