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Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles.

Publication ,  Journal Article
Williams, RS; Johnston, SA; Riedy, M; DeVit, MJ; McElligott, SG; Sanford, JC
Published in: Proc Natl Acad Sci U S A
April 1, 1991

Foreign genes were expressed in liver and skin cells of live mice by using a new apparatus to accelerate DNA-coated microprojectiles into tissues. After introduction of a plasmid in which the firefly luciferase gene was controlled by the human beta-actin promoter, luciferase activity was detectable for up to 14 days in mouse tissues (skin and liver). In situ hybridization histochemistry revealed that microprojectiles penetrated through multiple cell layers without evidence of tissue injury and that 10-20% of the cells in the bombarded area expressed the foreign gene. An advantage of the new design is that internal organs, such as liver, can be transfected without subjecting the tissue to a vacuum. This procedure potentially is applicable to a wide variety of tissues and cell types for studies of transcriptional control elements and for expression of foreign proteins in intact animals.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 1, 1991

Volume

88

Issue

7

Start / End Page

2726 / 2730

Location

United States

Related Subject Headings

  • Skin
  • Promoter Regions, Genetic
  • Plasmids
  • Organ Specificity
  • Nucleic Acid Hybridization
  • Mice, Inbred Strains
  • Mice
  • Luciferases
  • Liver
  • Genetic Engineering
 

Citation

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Williams, R. S., Johnston, S. A., Riedy, M., DeVit, M. J., McElligott, S. G., & Sanford, J. C. (1991). Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles. Proceedings of the National Academy of Sciences of the United States of America, 88(7), 2726–2730. https://doi.org/10.1073/pnas.88.7.2726
Williams, R. S., S. A. Johnston, M. Riedy, M. J. DeVit, S. G. McElligott, and J. C. Sanford. “Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles.Proceedings of the National Academy of Sciences of the United States of America 88, no. 7 (April 1991): 2726–30. https://doi.org/10.1073/pnas.88.7.2726.
Williams RS, Johnston SA, Riedy M, DeVit MJ, McElligott SG, Sanford JC. Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles. Proceedings of the National Academy of Sciences of the United States of America. 1991 Apr;88(7):2726–30.
Williams, R. S., et al. “Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles.Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 7, Apr. 1991, pp. 2726–30. Epmc, doi:10.1073/pnas.88.7.2726.
Williams RS, Johnston SA, Riedy M, DeVit MJ, McElligott SG, Sanford JC. Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles. Proceedings of the National Academy of Sciences of the United States of America. 1991 Apr;88(7):2726–2730.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 1, 1991

Volume

88

Issue

7

Start / End Page

2726 / 2730

Location

United States

Related Subject Headings

  • Skin
  • Promoter Regions, Genetic
  • Plasmids
  • Organ Specificity
  • Nucleic Acid Hybridization
  • Mice, Inbred Strains
  • Mice
  • Luciferases
  • Liver
  • Genetic Engineering