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Nuclear responses to depletion of mitochondrial DNA in human cells.

Publication ,  Journal Article
Li, K; Neufer, PD; Williams, RS
Published in: Am J Physiol
November 1995

The derivation of human cell lines devoid of mitochondrial (mt) DNA (rho 0) provides an opportunity to study nuclear responses to a chronic impairment of mitochondrial oxidative phosphorylation. Expression of several nuclear genes is induced in human rho 0 cells, including those encoding integral proteins of the mitochondrial inner membrane, intermediate filaments, and ribosomes. In contrast to conditions in which mitochondrial respiration is altered acutely, expression of heat shock proteins and immediate early genes is not induced. Mitochondria from rho 0 cells maintain a transmembrane electrochemical potential and are distributed within the cytoplasm of these cells in a manner indistinguishable from that of wild-type cells. We conclude that a chronic deficiency of mitochondrial oxidative phosphorylation produced by elimination of mtDNA is associated with a different pattern of gene induction than that provoked by other acute or subacute conditions that impair mitochondrial respiration or create energy demands in excess of mitochondrial respiratory capacity.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

November 1995

Volume

269

Issue

5 Pt 1

Start / End Page

C1265 / C1270

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Rhodamines
  • Rhodamine 123
  • RNA, Messenger
  • Oxidative Phosphorylation
  • Oligonucleotide Probes
  • Molecular Sequence Data
  • Microscopy, Fluorescence
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Li, K., Neufer, P. D., & Williams, R. S. (1995). Nuclear responses to depletion of mitochondrial DNA in human cells. Am J Physiol, 269(5 Pt 1), C1265–C1270. https://doi.org/10.1152/ajpcell.1995.269.5.C1265
Li, K., P. D. Neufer, and R. S. Williams. “Nuclear responses to depletion of mitochondrial DNA in human cells.Am J Physiol 269, no. 5 Pt 1 (November 1995): C1265–70. https://doi.org/10.1152/ajpcell.1995.269.5.C1265.
Li K, Neufer PD, Williams RS. Nuclear responses to depletion of mitochondrial DNA in human cells. Am J Physiol. 1995 Nov;269(5 Pt 1):C1265–70.
Li, K., et al. “Nuclear responses to depletion of mitochondrial DNA in human cells.Am J Physiol, vol. 269, no. 5 Pt 1, Nov. 1995, pp. C1265–70. Pubmed, doi:10.1152/ajpcell.1995.269.5.C1265.
Li K, Neufer PD, Williams RS. Nuclear responses to depletion of mitochondrial DNA in human cells. Am J Physiol. 1995 Nov;269(5 Pt 1):C1265–C1270.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

November 1995

Volume

269

Issue

5 Pt 1

Start / End Page

C1265 / C1270

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Rhodamines
  • Rhodamine 123
  • RNA, Messenger
  • Oxidative Phosphorylation
  • Oligonucleotide Probes
  • Molecular Sequence Data
  • Microscopy, Fluorescence
  • Humans