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Regulation of mitochondrial biogenesis in skeletal muscle by CaMK.

Publication ,  Journal Article
Wu, H; Kanatous, SB; Thurmond, FA; Gallardo, T; Isotani, E; Bassel-Duby, R; Williams, RS
Published in: Science
April 12, 2002

Endurance exercise training promotes mitochondrial biogenesis in skeletal muscle and enhances muscle oxidative capacity, but the signaling mechanisms involved are poorly understood. To investigate this adaptive process, we generated transgenic mice that selectively express in skeletal muscle a constitutively active form of calcium/calmodulin-dependent protein kinase IV (CaMKIV*). Skeletal muscles from these mice showed augmented mitochondrial DNA replication and mitochondrial biogenesis, up-regulation of mitochondrial enzymes involved in fatty acid metabolism and electron transport, and reduced susceptibility to fatigue during repetitive contractions. CaMK induced expression of peroxisome proliferator-activated receptor gamma coactivator 1 (PGC-1), a master regulator of mitochondrial biogenesis in vivo, and activated the PGC-1 gene promoter in cultured myocytes. Thus, a calcium-regulated signaling pathway controls mitochondrial biogenesis in mammalian cells.

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Published In

Science

DOI

EISSN

1095-9203

Publication Date

April 12, 2002

Volume

296

Issue

5566

Start / End Page

349 / 352

Location

United States

Related Subject Headings

  • Up-Regulation
  • Transgenes
  • Transcription Factors
  • Signal Transduction
  • Promoter Regions, Genetic
  • Oligonucleotide Array Sequence Analysis
  • Muscle, Skeletal
  • Muscle Fibers, Skeletal
  • Muscle Fatigue
  • Muscle Contraction
 

Citation

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Wu, H., Kanatous, S. B., Thurmond, F. A., Gallardo, T., Isotani, E., Bassel-Duby, R., & Williams, R. S. (2002). Regulation of mitochondrial biogenesis in skeletal muscle by CaMK. Science, 296(5566), 349–352. https://doi.org/10.1126/science.1071163
Wu, Hai, Shane B. Kanatous, Frederick A. Thurmond, Teresa Gallardo, Eiji Isotani, Rhonda Bassel-Duby, and R Sanders Williams. “Regulation of mitochondrial biogenesis in skeletal muscle by CaMK.Science 296, no. 5566 (April 12, 2002): 349–52. https://doi.org/10.1126/science.1071163.
Wu H, Kanatous SB, Thurmond FA, Gallardo T, Isotani E, Bassel-Duby R, et al. Regulation of mitochondrial biogenesis in skeletal muscle by CaMK. Science. 2002 Apr 12;296(5566):349–52.
Wu, Hai, et al. “Regulation of mitochondrial biogenesis in skeletal muscle by CaMK.Science, vol. 296, no. 5566, Apr. 2002, pp. 349–52. Pubmed, doi:10.1126/science.1071163.
Wu H, Kanatous SB, Thurmond FA, Gallardo T, Isotani E, Bassel-Duby R, Williams RS. Regulation of mitochondrial biogenesis in skeletal muscle by CaMK. Science. 2002 Apr 12;296(5566):349–352.
Journal cover image

Published In

Science

DOI

EISSN

1095-9203

Publication Date

April 12, 2002

Volume

296

Issue

5566

Start / End Page

349 / 352

Location

United States

Related Subject Headings

  • Up-Regulation
  • Transgenes
  • Transcription Factors
  • Signal Transduction
  • Promoter Regions, Genetic
  • Oligonucleotide Array Sequence Analysis
  • Muscle, Skeletal
  • Muscle Fibers, Skeletal
  • Muscle Fatigue
  • Muscle Contraction