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Effects of experimental diabetes on adrenergic and cholinergic receptors of rat myocardium.

Publication ,  Journal Article
Williams, RS; Schaible, TF; Scheuer, J; Kennedy, R
Published in: Diabetes
October 1983

To more fully characterize the alterations in myocardial adrenergic and cholinergic receptors induced by the diabetic state, we investigated the binding characteristics of (--) [3H] dihydroalprenolol to beta adrenergic receptors (bAR), [3H] prazosin to alpha adrenergic receptors (aAR), and [3H] quinuclidinyl-benzilate to muscarinic cholinergic receptors (MCR) in myocardial membranes derived from rats 8 wk after treatment with streptozotocin. We also studied an equal number of animals from three control groups: free-eating nondiabetics, pair-weighted nondiabetics, and streptozotocin-treated animals treated daily with insulin. Diabetic hearts demonstrated 27% fewer bAR (P less than 0.01) and 31% fewer aAR (P less than 0.01) than free-eating controls, without changes in MCR, and without changes in antagonist affinity, agonist affinity, or agonist slope factor (pseudo-Hill coefficient) for any class of receptors. Food restriction had no effect on receptor characteristics, and treatment of diabetic rats with insulin prevented any downregulation of cardiac bAR or aAR. The parallel decrease in both bAR and aAR suggests that streptozotocin-induced hypothyroidism is not the primary causative factor of bAR downregulation in this model, since hypothyroidism produces upregulation of aAR. Furthermore, the lack of change in cardiac MCR suggests that the adrenergic receptor alterations are not the result of nonspecific abnormalities of protein synthesis in the diabetic heart. Further studies are required to establish the physiologic significance of these receptor alterations, but these data support the hypothesis that altered adrenergic receptor properties may underlie, at least in part, the chronotropic and inotropic abnormalities of cardiac performance that are associated with the diabetic state.

Duke Scholars

Published In

Diabetes

DOI

ISSN

0012-1797

Publication Date

October 1983

Volume

32

Issue

10

Start / End Page

881 / 886

Location

United States

Related Subject Headings

  • Receptors, Muscarinic
  • Receptors, Cholinergic
  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic, alpha
  • Rats, Inbred Strains
  • Rats
  • Quinuclidinyl Benzilate
  • Prazosin
  • Organ Size
  • Myocardium
 

Citation

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ICMJE
MLA
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Williams, R. S., Schaible, T. F., Scheuer, J., & Kennedy, R. (1983). Effects of experimental diabetes on adrenergic and cholinergic receptors of rat myocardium. Diabetes, 32(10), 881–886. https://doi.org/10.2337/diab.32.10.881
Williams, R. S., T. F. Schaible, J. Scheuer, and R. Kennedy. “Effects of experimental diabetes on adrenergic and cholinergic receptors of rat myocardium.Diabetes 32, no. 10 (October 1983): 881–86. https://doi.org/10.2337/diab.32.10.881.
Williams RS, Schaible TF, Scheuer J, Kennedy R. Effects of experimental diabetes on adrenergic and cholinergic receptors of rat myocardium. Diabetes. 1983 Oct;32(10):881–6.
Williams, R. S., et al. “Effects of experimental diabetes on adrenergic and cholinergic receptors of rat myocardium.Diabetes, vol. 32, no. 10, Oct. 1983, pp. 881–86. Pubmed, doi:10.2337/diab.32.10.881.
Williams RS, Schaible TF, Scheuer J, Kennedy R. Effects of experimental diabetes on adrenergic and cholinergic receptors of rat myocardium. Diabetes. 1983 Oct;32(10):881–886.

Published In

Diabetes

DOI

ISSN

0012-1797

Publication Date

October 1983

Volume

32

Issue

10

Start / End Page

881 / 886

Location

United States

Related Subject Headings

  • Receptors, Muscarinic
  • Receptors, Cholinergic
  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic, alpha
  • Rats, Inbred Strains
  • Rats
  • Quinuclidinyl Benzilate
  • Prazosin
  • Organ Size
  • Myocardium