Transient expression of a winged-helix protein, MNF-beta, during myogenesis.
Journal Article (Journal Article)
A novel winged-helix transcription factor, MNF-beta, is expressed coincidentally with cell cycle withdrawal and differentiation of skeletal myogenic cells. MNF-beta is closely related to the myocyte nuclear factor (MNF) protein previously described (now termed MNF-alpha), but expression of the two isoforms is differentially regulated, and they exhibit distinctive functional properties with respect to DNA binding in vitro and transcriptional regulatory activity in transient-transfection assays. A DNA sequence motif binding MNF-beta with high affinity was selected from a library of random oligonucleotides and was found to be similar to but distinct from the cognate binding site for HNF-3beta, a more distantly related winged-helix protein. The temporal pattern of MNF-beta expression and the presence of MNF binding motifs within conserved promoter elements of several genes that modulate cell cycle progression support a working hypothesis that MNF proteins may modulate proliferation of myogenic precursor cells during development and muscle regeneration.
Full Text
Duke Authors
Cited Authors
- Yang, Q; Bassel-Duby, R; Williams, RS
Published Date
- September 1997
Published In
Volume / Issue
- 17 / 9
Start / End Page
- 5236 - 5243
PubMed ID
- 9271401
Pubmed Central ID
- PMC232374
International Standard Serial Number (ISSN)
- 0270-7306
Digital Object Identifier (DOI)
- 10.1128/MCB.17.9.5236
Language
- eng
Conference Location
- United States