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Targeted inhibition of calcineurin in pressure-overload cardiac hypertrophy. Preservation of systolic function.

Publication ,  Journal Article
Hill, JA; Rothermel, B; Yoo, K-D; Cabuay, B; Demetroulis, E; Weiss, RM; Kutschke, W; Bassel-Duby, R; Williams, RS
Published in: J Biol Chem
March 22, 2002

Calcineurin is a Ca(2+)/calmodulin-activated protein phosphatase that transduces hypertrophic stimuli to regulate transcriptional control of myocyte transformation. It is not known whether overexpression of MCIP1, a recently described endogenous inhibitor of calcineurin, impacts the hypertrophic response to pathophysiologically relevant pressure overload. Further, the functional consequences of calcineurin inhibition by MCIP1 under conditions of hemodynamic stress are unknown. Transgenic mice expressing a human cDNA encoding hMCIP1 in the myocardium were subjected to thoracic aortic banding. Transgenic mice and wild type littermates tolerated pressure overload equally well. Wild type mice developed left ventricular hypertrophy, but the hypertrophic response in transgenics was significantly blunted. An isoform of MCIP1 transcript was up-regulated by pressure stress, whereas MCIP2 transcript was not. Expression patterns of fetal genes were differentially regulated in banded MCIP1 hearts compared with wild type. Echocardiography performed at 3 weeks and 3 months revealed preservation of both left ventricular size and systolic function in banded MCIP1 mice despite the attenuated hypertrophic response. These data demonstrate attenuation of hypertrophic transformation when calcineurin is inhibited by MCIP1. Further, these data suggest that activation of hypertrophic marker genes may not be directly dependent on calcineurin activity. Finally, they demonstrate that ventricular performance is preserved despite attenuation of compensatory hypertrophy.

Duke Scholars

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

March 22, 2002

Volume

277

Issue

12

Start / End Page

10251 / 10255

Location

United States

Related Subject Headings

  • Up-Regulation
  • Time Factors
  • RNA, Messenger
  • RNA
  • Protein Isoforms
  • Protein Binding
  • Pressure
  • Myocardium
  • Mice, Transgenic
  • Mice
 

Citation

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Hill, J. A., Rothermel, B., Yoo, K.-D., Cabuay, B., Demetroulis, E., Weiss, R. M., … Williams, R. S. (2002). Targeted inhibition of calcineurin in pressure-overload cardiac hypertrophy. Preservation of systolic function. J Biol Chem, 277(12), 10251–10255. https://doi.org/10.1074/jbc.M110722200
Hill, Joseph A., Beverly Rothermel, Ki-Dong Yoo, Barry Cabuay, Elaine Demetroulis, Robert M. Weiss, William Kutschke, Rhonda Bassel-Duby, and R Sanders Williams. “Targeted inhibition of calcineurin in pressure-overload cardiac hypertrophy. Preservation of systolic function.J Biol Chem 277, no. 12 (March 22, 2002): 10251–55. https://doi.org/10.1074/jbc.M110722200.
Hill JA, Rothermel B, Yoo K-D, Cabuay B, Demetroulis E, Weiss RM, et al. Targeted inhibition of calcineurin in pressure-overload cardiac hypertrophy. Preservation of systolic function. J Biol Chem. 2002 Mar 22;277(12):10251–5.
Hill, Joseph A., et al. “Targeted inhibition of calcineurin in pressure-overload cardiac hypertrophy. Preservation of systolic function.J Biol Chem, vol. 277, no. 12, Mar. 2002, pp. 10251–55. Pubmed, doi:10.1074/jbc.M110722200.
Hill JA, Rothermel B, Yoo K-D, Cabuay B, Demetroulis E, Weiss RM, Kutschke W, Bassel-Duby R, Williams RS. Targeted inhibition of calcineurin in pressure-overload cardiac hypertrophy. Preservation of systolic function. J Biol Chem. 2002 Mar 22;277(12):10251–10255.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

March 22, 2002

Volume

277

Issue

12

Start / End Page

10251 / 10255

Location

United States

Related Subject Headings

  • Up-Regulation
  • Time Factors
  • RNA, Messenger
  • RNA
  • Protein Isoforms
  • Protein Binding
  • Pressure
  • Myocardium
  • Mice, Transgenic
  • Mice