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Sequence elements required for transcriptional activity of the human myoglobin promoter in intact myocardium.

Publication ,  Journal Article
Bassel-Duby, R; Grohe, CM; Jessen, ME; Parsons, WJ; Richardson, JA; Chao, R; Grayson, J; Ring, WS; Williams, RS
Published in: Circ Res
August 1993

To define sequence elements required for myoglobin gene transcription in the intact heart, we examined the expression of a reporter gene under the control of a 380-bp upstream segment (-373 to +7) from the human myoglobin gene in transgenic mouse embryos and after gene transfer into left ventricular myocardium of adult rats. This proximal upstream region was sufficient to direct expression of luciferase selectively in cardiac and skeletal muscle of mouse embryos and to recapitulate the pattern of expression of the endogenous mouse myoglobin gene. This same upstream region was transcriptionally active after injection of plasmid DNA into the left ventricular wall of adult rats. Point mutations within two evolutionarily conserved sequence elements--a cytosine-rich (CCAC-box) motif and an A+T-rich (A/T) motif--severely impaired transcription within the intact heart. Nuclear extracts from neonatal cardiomyocytes contain protein factors that bind to each of these elements in a sequence-specific manner. We conclude that combinatorial interactions between the cognate DNA binding factors that recognize these motifs are necessary for transcriptional activity of the myoglobin upstream region in cardiac muscle.

Duke Scholars

Published In

Circ Res

DOI

ISSN

0009-7330

Publication Date

August 1993

Volume

73

Issue

2

Start / End Page

360 / 366

Location

United States

Related Subject Headings

  • Transfection
  • Transcription, Genetic
  • Rats, Sprague-Dawley
  • Rats
  • Promoter Regions, Genetic
  • Oocytes
  • Myoglobin
  • Mutation
  • Molecular Sequence Data
  • Mice, Transgenic
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bassel-Duby, R., Grohe, C. M., Jessen, M. E., Parsons, W. J., Richardson, J. A., Chao, R., … Williams, R. S. (1993). Sequence elements required for transcriptional activity of the human myoglobin promoter in intact myocardium. Circ Res, 73(2), 360–366. https://doi.org/10.1161/01.res.73.2.360
Bassel-Duby, R., C. M. Grohe, M. E. Jessen, W. J. Parsons, J. A. Richardson, R. Chao, J. Grayson, W. S. Ring, and R. S. Williams. “Sequence elements required for transcriptional activity of the human myoglobin promoter in intact myocardium.Circ Res 73, no. 2 (August 1993): 360–66. https://doi.org/10.1161/01.res.73.2.360.
Bassel-Duby R, Grohe CM, Jessen ME, Parsons WJ, Richardson JA, Chao R, et al. Sequence elements required for transcriptional activity of the human myoglobin promoter in intact myocardium. Circ Res. 1993 Aug;73(2):360–6.
Bassel-Duby, R., et al. “Sequence elements required for transcriptional activity of the human myoglobin promoter in intact myocardium.Circ Res, vol. 73, no. 2, Aug. 1993, pp. 360–66. Pubmed, doi:10.1161/01.res.73.2.360.
Bassel-Duby R, Grohe CM, Jessen ME, Parsons WJ, Richardson JA, Chao R, Grayson J, Ring WS, Williams RS. Sequence elements required for transcriptional activity of the human myoglobin promoter in intact myocardium. Circ Res. 1993 Aug;73(2):360–366.

Published In

Circ Res

DOI

ISSN

0009-7330

Publication Date

August 1993

Volume

73

Issue

2

Start / End Page

360 / 366

Location

United States

Related Subject Headings

  • Transfection
  • Transcription, Genetic
  • Rats, Sprague-Dawley
  • Rats
  • Promoter Regions, Genetic
  • Oocytes
  • Myoglobin
  • Mutation
  • Molecular Sequence Data
  • Mice, Transgenic