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Myocyte nuclear factor, a novel winged-helix transcription factor under both developmental and neural regulation in striated myocytes.

Publication ,  Journal Article
Bassel-Duby, R; Hernandez, MD; Yang, Q; Rochelle, JM; Seldin, MF; Williams, RS
Published in: Mol Cell Biol
July 1994

A sequence motif (CCAC box) within an upstream enhancer region of the human myoglobin gene is essential for transcriptional activity in both cardiac and skeletal muscle. A cDNA clone, myocyte nuclear factor (MNF), was isolated from a murine expression library on the basis of sequence-specific binding to the myoglobin CCAC box motif and was found to encode a novel member of the winged-helix or HNF-3/fork head family of transcription factors. Probes based on this sequence identify two mRNA species that are upregulated during myocyte differentiation, and antibodies raised against recombinant MNF identify proteins of approximately 90, 68, and 65 kDa whose expression is regulated following differentiation of myogenic cells in culture. In addition, the 90-kDa form of MNF is phosphorylated and is upregulated in intact muscles subjected to chronic motor nerve stimulation, a potent stimulus to myoglobin gene regulation. Amino acid residues 280 to 389 of MNF demonstrate 35 to 89% sequence identity to the winged-helix domain from other known members of this family, but MNF is otherwise divergent. A proline-rich amino-terminal region (residues 1 to 206) of MNF functions as a transcriptional activation domain. These studies provide the first evidence that members of the winged-helix family of transcription factors have a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli.

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

July 1994

Volume

14

Issue

7

Start / End Page

4596 / 4605

Location

United States

Related Subject Headings

  • Transcription Factors
  • Recombinant Fusion Proteins
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Oligonucleotide Probes
  • Myoglobin
  • Muscles
  • Muridae
  • Molecular Sequence Data
  • Mice, Inbred C3H
 

Citation

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MLA
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Bassel-Duby, R., Hernandez, M. D., Yang, Q., Rochelle, J. M., Seldin, M. F., & Williams, R. S. (1994). Myocyte nuclear factor, a novel winged-helix transcription factor under both developmental and neural regulation in striated myocytes. Mol Cell Biol, 14(7), 4596–4605. https://doi.org/10.1128/mcb.14.7.4596-4605.1994
Bassel-Duby, R., M. D. Hernandez, Q. Yang, J. M. Rochelle, M. F. Seldin, and R. S. Williams. “Myocyte nuclear factor, a novel winged-helix transcription factor under both developmental and neural regulation in striated myocytes.Mol Cell Biol 14, no. 7 (July 1994): 4596–4605. https://doi.org/10.1128/mcb.14.7.4596-4605.1994.
Bassel-Duby R, Hernandez MD, Yang Q, Rochelle JM, Seldin MF, Williams RS. Myocyte nuclear factor, a novel winged-helix transcription factor under both developmental and neural regulation in striated myocytes. Mol Cell Biol. 1994 Jul;14(7):4596–605.
Bassel-Duby, R., et al. “Myocyte nuclear factor, a novel winged-helix transcription factor under both developmental and neural regulation in striated myocytes.Mol Cell Biol, vol. 14, no. 7, July 1994, pp. 4596–605. Pubmed, doi:10.1128/mcb.14.7.4596-4605.1994.
Bassel-Duby R, Hernandez MD, Yang Q, Rochelle JM, Seldin MF, Williams RS. Myocyte nuclear factor, a novel winged-helix transcription factor under both developmental and neural regulation in striated myocytes. Mol Cell Biol. 1994 Jul;14(7):4596–4605.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

July 1994

Volume

14

Issue

7

Start / End Page

4596 / 4605

Location

United States

Related Subject Headings

  • Transcription Factors
  • Recombinant Fusion Proteins
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Oligonucleotide Probes
  • Myoglobin
  • Muscles
  • Muridae
  • Molecular Sequence Data
  • Mice, Inbred C3H