Persistent expression of MNF identifies myogenic stem cells in postnatal muscles.

Journal Article

Skeletal muscles contain an undifferentiated myogenic stem cell pool (satellite cells) that can be mobilized to regenerate myofibers in response to injury. We have determined that the winged helix transcription factor MNF is expressed selectively in quiescent satellite cells, which do not express known regulators of the myogenic program. Following muscle injury, MNF is present transiently in proliferating satellite cells and in centralized nuclei of regenerating myofibers, but expression declines as these fibers mature, until only the residual stem cell pool continues to express detectable levels of MNF. MNF also is expressed selectively but transiently at embryonic stages of myogenesis in the developing myotome, limb bud precursors, and heart tube, but by late fetal stages of development, MNF is down-regulated within differentiated cardiac and skeletal myocytes, and persistently high expression is observed only in satellite cells. These data identify MNF as a marker of quiescent satellite cells and suggest that downstream genes controlled by MNF serve to modulate proliferative growth or differentiation in this unique cell population.

Full Text

Duke Authors

Cited Authors

  • Garry, DJ; Yang, Q; Bassel-Duby, R; Williams, RS

Published Date

  • August 15, 1997

Published In

Volume / Issue

  • 188 / 2

Start / End Page

  • 280 - 294

PubMed ID

  • 9268575

International Standard Serial Number (ISSN)

  • 0012-1606

Digital Object Identifier (DOI)

  • 10.1006/dbio.1997.8657

Language

  • eng

Conference Location

  • United States