The winged-helix/forkhead protein myocyte nuclear factor beta (MNF-beta) forms a co-repressor complex with mammalian sin3B.

Published

Journal Article

Winged-helix/forkhead proteins regulate developmental events in both invertebrate and vertebrate organisms, but biochemical functions that establish a mechanism of action have been defined for only a few members of this extensive gene family. Here we demonstrate that MNF (myocyte nuclear factor)-beta, a winged-helix protein expressed selectively and transiently in myogenic precursor cells of the heart and skeletal muscles, collaborates with proteins of the mammalian Sin3 (mSin3) family to repress transcription. Mutated forms of MNF-beta that fail to bind mSin3 are defective in transcriptional repression and in negative growth regulation, an overexpression phenotype revealed in oncogenic transformation assays. These data extend the known repertoire of transcription factors with which mSin3 proteins can function as co-repressors to include members of the winged-helix gene family. Transcriptional repression by MNF-beta-mSin3 complexes may contribute to the co-ordination of cellular proliferation and terminal differentiation of myogenic precursor cells.

Full Text

Duke Authors

Cited Authors

  • Yang, Q; Kong, Y; Rothermel, B; Garry, DJ; Bassel-Duby, R; Williams, RS

Published Date

  • January 15, 2000

Published In

Volume / Issue

  • 345 Pt 2 /

Start / End Page

  • 335 - 343

PubMed ID

  • 10620510

Pubmed Central ID

  • 10620510

International Standard Serial Number (ISSN)

  • 0264-6021

Language

  • eng

Conference Location

  • England