Induction of immediate-early genes by angiotensin II and endothelin-1 in adult rat cardiomyocytes.

Published

Journal Article

OBJECTIVE: Few molecular signals for induction of myocardial hypertrophy have been identified. This study was carried out to investigate the action of angiotensin II and endothelin on the growth- and differentiation-related genes Egr-1 (early growth response gene 1) and c-fos in isolated adult rat cardiomyocytes. METHODS: Cardiac myocytes from male Wistar-Kyoto rats were isolated and incubated with angiotensin II and endothelin-1 in Dulbecco's modified Eagle's medium. RNA was isolated and blotted, and densitometric analysis was performed. All experiments were repeated at least three times. RESULTS: Endothelin-1 (10(-7) mmol/l) induced a 20-25-fold rise in Egr-1 messenger RNA within 15 min. This effect was dose-dependent. c-fos was induced 10-20-fold within 15 min with similar dose-response characteristics. Angiotensin II also induced Egr-1 and c-fos with kinetics similar to endothelin but a cofactor from fetal calf serum was needed for full c-fos expression. The protein kinase C activator phorbol 12-myristate 13-acetate also induced Egr-1. CONCLUSIONS: The results identify Egr-1 and c-fos as target genes for the action of endothelin and angiotensin II in the adult myocardium suggesting that induction of the genes may be part of the signal transduction pathway for angiotensin II and endothelin in the myocardium.

Full Text

Duke Authors

Cited Authors

  • Neyses, L; Nouskas, J; Luyken, J; Fronhoffs, S; Oberdorf, S; Pfeifer, U; Williams, RS; Sukhatme, VP; Vetter, H

Published Date

  • September 1, 1993

Published In

Volume / Issue

  • 11 / 9

Start / End Page

  • 927 - 934

PubMed ID

  • 7504706

Pubmed Central ID

  • 7504706

International Standard Serial Number (ISSN)

  • 0263-6352

Digital Object Identifier (DOI)

  • 10.1097/00004872-199309000-00006

Language

  • eng

Conference Location

  • England