Synthesis and characterization of a ruthenium(II)-based redox conjugate for reagentless biosensing.
Published
Journal Article
Synthesis of a novel sulfhydryl-specific, tetraammine Ru(II)polypyridyl complex, [Ru(II)(NH(3))(4)(1,10-phenanthroline-5-maleimide)](PF(6))(2), which exhibits environment-sensitive electrochemical properties is described. When conjugated to an allosteric site in a genetically engineered mutant of maltose binding protein, the formal potential of the conjugated redox probe is shifted to higher potential upon maltose binding. The magnitude of this potential shift was used to measure maltose affinity of the protein-redox conjugate complex and to monitor maltose concentration in solution. These results are presented in context of reagentless biosensing.
Full Text
Duke Authors
Cited Authors
- Trammell, SA; Goldston, HM; Tran, PT; Tender, LM; Conrad, DW; Benson, DE; Hellinga, HW
Published Date
- July 2001
Published In
Volume / Issue
- 12 / 4
Start / End Page
- 643 - 647
PubMed ID
- 11459471
Pubmed Central ID
- 11459471
International Standard Serial Number (ISSN)
- 1043-1802
Digital Object Identifier (DOI)
- 10.1021/bc010022q
Language
- eng
Conference Location
- United States