Synthesis and characterization of a ruthenium(II)-based redox conjugate for reagentless biosensing.

Published

Journal Article

Synthesis of a novel sulfhydryl-specific, tetraammine Ru(II)polypyridyl complex, [Ru(II)(NH(3))(4)(1,10-phenanthroline-5-maleimide)](PF(6))(2), which exhibits environment-sensitive electrochemical properties is described. When conjugated to an allosteric site in a genetically engineered mutant of maltose binding protein, the formal potential of the conjugated redox probe is shifted to higher potential upon maltose binding. The magnitude of this potential shift was used to measure maltose affinity of the protein-redox conjugate complex and to monitor maltose concentration in solution. These results are presented in context of reagentless biosensing.

Full Text

Duke Authors

Cited Authors

  • Trammell, SA; Goldston, HM; Tran, PT; Tender, LM; Conrad, DW; Benson, DE; Hellinga, HW

Published Date

  • July 2001

Published In

Volume / Issue

  • 12 / 4

Start / End Page

  • 643 - 647

PubMed ID

  • 11459471

Pubmed Central ID

  • 11459471

International Standard Serial Number (ISSN)

  • 1043-1802

Digital Object Identifier (DOI)

  • 10.1021/bc010022q

Language

  • eng

Conference Location

  • United States