Skip to main content
Journal cover image

Developmental expression of the glucose-dependent insulinotropic polypeptide gene in rat intestine.

Publication ,  Journal Article
Higashimoto, Y; Liddle, RA
Published in: Biochem Biophys Res Commun
June 15, 1994

The developmental expression of the glucose-dependent insulinotropic polypeptide (GIP) gene was investigated in rat intestine. Steady state levels of GIP mRNA were determined in the intestine during fetal and postnatal development by double ribonuclease protection assays. GIP mRNA could be detected as early as day 20 of embryonic development and very low levels remained until postnatal day 3. The GIP mRNA levels increased markedly in the period between days 3 and 5 of postnatal life and then gradually increased toward adult levels. Since intron 1 of the GIP gene contains putative TATA and CCAAT boxes, and some potential cis-acting promoter elements, we examined whether or not another transcript starting from exon 2 of the GIP gene is expressed during development of rat intestine. Ribonuclease protection assays suggested that although an abbreviated transcript might exist starting from exon 2, it appears to be minor and its relative abundance is unchanged during development or following intraduodenal glucose stimulation. These observations suggest that GIP may play an important role in early postnatal development probably associated with suckling.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

June 15, 1994

Volume

201

Issue

2

Start / End Page

964 / 972

Location

United States

Related Subject Headings

  • TATA Box
  • Ribonucleases
  • Rats, Wistar
  • Rats
  • RNA, Messenger
  • RNA Probes
  • Promoter Regions, Genetic
  • Muscle, Smooth
  • Muscle Development
  • Intestine, Small
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Higashimoto, Y., & Liddle, R. A. (1994). Developmental expression of the glucose-dependent insulinotropic polypeptide gene in rat intestine. Biochem Biophys Res Commun, 201(2), 964–972. https://doi.org/10.1006/bbrc.1994.1796
Higashimoto, Y., and R. A. Liddle. “Developmental expression of the glucose-dependent insulinotropic polypeptide gene in rat intestine.Biochem Biophys Res Commun 201, no. 2 (June 15, 1994): 964–72. https://doi.org/10.1006/bbrc.1994.1796.
Higashimoto Y, Liddle RA. Developmental expression of the glucose-dependent insulinotropic polypeptide gene in rat intestine. Biochem Biophys Res Commun. 1994 Jun 15;201(2):964–72.
Higashimoto, Y., and R. A. Liddle. “Developmental expression of the glucose-dependent insulinotropic polypeptide gene in rat intestine.Biochem Biophys Res Commun, vol. 201, no. 2, June 1994, pp. 964–72. Pubmed, doi:10.1006/bbrc.1994.1796.
Higashimoto Y, Liddle RA. Developmental expression of the glucose-dependent insulinotropic polypeptide gene in rat intestine. Biochem Biophys Res Commun. 1994 Jun 15;201(2):964–972.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

June 15, 1994

Volume

201

Issue

2

Start / End Page

964 / 972

Location

United States

Related Subject Headings

  • TATA Box
  • Ribonucleases
  • Rats, Wistar
  • Rats
  • RNA, Messenger
  • RNA Probes
  • Promoter Regions, Genetic
  • Muscle, Smooth
  • Muscle Development
  • Intestine, Small