Relative bioactivities of cholecystokinins-8 and -33 on rat pancreatic acini.

Published

Journal Article

The relative potencies of cholecystokinin (CCK-33) and its carboxyl terminal octapeptide (CCK-8) for stimulation of amylase release from rat pancreatic acini was measured. Porcine CCK-33 and synthetic CCK-8 were initially subjected to high pressure liquid chromatography to assess purity. Concentrations of each peptide were determined by amino acid analysis. The relative immunoreactivities of CCK-33 and CCK-8 were compared using an antibody that recognizes the common carboxyl terminus of these forms. This antibody bound CCK-8 and CCK-33 with nearly equal affinity. The relative potencies of CCK-33 and CCK-8 were then measured by comparing their abilities to stimulate amylase release from isolated rat pancreatic acini. Statistical analysis of the relative potencies of the two hormones indicated that CCK-8 was 36% more potent than CCK-33 in this assay system. These data suggest that differences in biological activities between large and small forms of CCK are not as great as previously reported.

Full Text

Duke Authors

Cited Authors

  • Liddle, RA; Elashoff, J; Reeve, JR

Published Date

  • September 1986

Published In

Volume / Issue

  • 7 / 5

Start / End Page

  • 723 - 727

PubMed ID

  • 2432584

Pubmed Central ID

  • 2432584

Electronic International Standard Serial Number (EISSN)

  • 1873-5169

International Standard Serial Number (ISSN)

  • 0079-0753

Digital Object Identifier (DOI)

  • 10.1016/0196-9781(86)90085-9

Language

  • eng