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Molecular cloning of rat glucose-dependent insulinotropic peptide (GIP).

Publication ,  Journal Article
Higashimoto, Y; Simchock, J; Liddle, RA
Published in: Biochim Biophys Acta
August 17, 1992

A cDNA clone encoding glucose-dependent insulinotropic peptide (GIP) was identified that consisted of 34 bp of 5' untranslated sequence, an open reading frame of 432 bp and 115 bp in the 3' untranslated region. The deduced amino acid sequence revealed a 144 amino acid preprohormone consisting of a 43 amino acid N-terminal extension including a signal peptide, a 42 amino acid hormone, and a 59 amino acid C-terminal extension. Rat GIP differs from the human hormone by two amino acid substitutions: arginine for histidine at position 18 and leucine for isoleucine at position 40. A single mRNA from small intestine of approximately 800 bases was identified on Northern blot analysis in equivalent amounts in proximal and distal small intestine.

Duke Scholars

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

August 17, 1992

Volume

1132

Issue

1

Start / End Page

72 / 74

Location

Netherlands

Related Subject Headings

  • Transcription, Genetic
  • Rats
  • RNA, Messenger
  • RNA
  • Polymerase Chain Reaction
  • Poly A
  • Open Reading Frames
  • Oligodeoxyribonucleotides
  • Molecular Sequence Data
  • Intestinal Mucosa
 

Citation

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MLA
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Higashimoto, Y., Simchock, J., & Liddle, R. A. (1992). Molecular cloning of rat glucose-dependent insulinotropic peptide (GIP). Biochim Biophys Acta, 1132(1), 72–74. https://doi.org/10.1016/0167-4781(92)90054-4
Higashimoto, Y., J. Simchock, and R. A. Liddle. “Molecular cloning of rat glucose-dependent insulinotropic peptide (GIP).Biochim Biophys Acta 1132, no. 1 (August 17, 1992): 72–74. https://doi.org/10.1016/0167-4781(92)90054-4.
Higashimoto Y, Simchock J, Liddle RA. Molecular cloning of rat glucose-dependent insulinotropic peptide (GIP). Biochim Biophys Acta. 1992 Aug 17;1132(1):72–4.
Higashimoto, Y., et al. “Molecular cloning of rat glucose-dependent insulinotropic peptide (GIP).Biochim Biophys Acta, vol. 1132, no. 1, Aug. 1992, pp. 72–74. Pubmed, doi:10.1016/0167-4781(92)90054-4.
Higashimoto Y, Simchock J, Liddle RA. Molecular cloning of rat glucose-dependent insulinotropic peptide (GIP). Biochim Biophys Acta. 1992 Aug 17;1132(1):72–74.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

August 17, 1992

Volume

1132

Issue

1

Start / End Page

72 / 74

Location

Netherlands

Related Subject Headings

  • Transcription, Genetic
  • Rats
  • RNA, Messenger
  • RNA
  • Polymerase Chain Reaction
  • Poly A
  • Open Reading Frames
  • Oligodeoxyribonucleotides
  • Molecular Sequence Data
  • Intestinal Mucosa