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Isolation and characterization of the gene encoding rat glucose-dependent insulinotropic peptide.

Publication ,  Journal Article
Higashimoto, Y; Liddle, RA
Published in: Biochem Biophys Res Commun
May 28, 1993

The rat glucose-dependent insulinotropic peptide (GIP) gene has been isolated and characterized. The gene spans approximately 8.2 kilobase pairs (kb) and the GIP mRNA (0.8 kb) is encoded by six exons. The 42 amino acid hormone is encoded by exons 3 and 4. The exon-intron organization of the rat GIP gene revealed that the splice acceptor site for intron 2 is 24 nucleotides downstream compared to the comparable splice acceptor site in the human gene. This intron sliding results in an 8 amino acid deletion in the amino terminal extension of the prepropeptide. Primer extension analysis and RNase protection assay demonstrated the existence of multiple closely spaced sites for transcriptional initiation. Both the 5'-flanking region and intron 1 contain TATA and CCAAT boxes consistent with initiation of gene transcription, although a TATA box in intron 1 is functionally inactive in adult rats in spite of its reasonable location.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

May 28, 1993

Volume

193

Issue

1

Start / End Page

182 / 190

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Sequence Homology, Amino Acid
  • Rats
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Introns
  • Humans
  • Gastric Inhibitory Polypeptide
  • Exons
  • DNA
 

Citation

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Higashimoto, Y., & Liddle, R. A. (1993). Isolation and characterization of the gene encoding rat glucose-dependent insulinotropic peptide. Biochem Biophys Res Commun, 193(1), 182–190. https://doi.org/10.1006/bbrc.1993.1607
Higashimoto, Y., and R. A. Liddle. “Isolation and characterization of the gene encoding rat glucose-dependent insulinotropic peptide.Biochem Biophys Res Commun 193, no. 1 (May 28, 1993): 182–90. https://doi.org/10.1006/bbrc.1993.1607.
Higashimoto Y, Liddle RA. Isolation and characterization of the gene encoding rat glucose-dependent insulinotropic peptide. Biochem Biophys Res Commun. 1993 May 28;193(1):182–90.
Higashimoto, Y., and R. A. Liddle. “Isolation and characterization of the gene encoding rat glucose-dependent insulinotropic peptide.Biochem Biophys Res Commun, vol. 193, no. 1, May 1993, pp. 182–90. Pubmed, doi:10.1006/bbrc.1993.1607.
Higashimoto Y, Liddle RA. Isolation and characterization of the gene encoding rat glucose-dependent insulinotropic peptide. Biochem Biophys Res Commun. 1993 May 28;193(1):182–190.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

May 28, 1993

Volume

193

Issue

1

Start / End Page

182 / 190

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Sequence Homology, Amino Acid
  • Rats
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Introns
  • Humans
  • Gastric Inhibitory Polypeptide
  • Exons
  • DNA