Expression of differentiation antigens by hybrids of human lymphoblastoid cells.
Journal Article (Journal Article)
In previous communications, we have described the expression of class I and class II histocompatibility antigens by hybrids of human B and T lymphoblastoid cell lines (B- and T-LCL). In all cases, such hybrids were found to resemble their B-LCL parents, expressing high levels of class I and class II antigens encoded by both parent cell lines. In the current study, we have conducted a more extensive analysis of B-LCLxT-LCL hybrids with a panel of monoclonal antibodies recognizing a variety of B and T lymphocyte differentiation markers. Rather than exhibiting a B-LCL-dominant phenotype, most hybrids were found to express a majority of both T and B lymphocyte antigens expressed by their parent cell lines. Several hybrids of pairs of dissimilar T-LCL were also produced and analyzed. Again, a majority of parental antigens was expressed on the hybrids. However, eight of eight hybrids of the T-LCL CEM and HSB failed to express HNK-1, an antigen strongly expressed by HSB; and two hybrids of the T-LCL CEM and SKW3 expressed CD3, an antigen expressed by neither parent cell line.
Full Text
Duke Authors
Cited Authors
- Howell, DN; Salter, RD; Koppelman, BD; Cresswell, P
Published Date
- December 1986
Published In
Volume / Issue
- 17 / 4
Start / End Page
- 443 - 455
PubMed ID
- 3098709
Pubmed Central ID
- 3098709
International Standard Serial Number (ISSN)
- 0198-8859
Digital Object Identifier (DOI)
- 10.1016/0198-8859(86)90303-4
Language
- eng
Conference Location
- United States