Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays.

Published

Journal Article

Nucleocapsids in quantities approaching 1 mg were purified from 109 measles virus-infected cells. They contained one polypeptide species with a molecular weight of 59,000. Antiserum was raised in rabbits against purified nucleocapsids and used in a competitive radioimmunoassay. Because of their instability, purified nucleocapsids were not suitable for use in such an assay. Instead partially purified nucleocapsids from HEp-2 cells persistently infected with measles virus and labeled in vitro with [35S]methionine were used as the source of radioactive antigen. The radioimmunoassay thus developed measured less than 5 ng of nucleocapsids in infected cells.

Full Text

Duke Authors

Cited Authors

  • Moore, PM; Hayes, EC; Miller, SE; Wright, LL; Machamer, CE; Zweerink, HJ

Published Date

  • June 1978

Published In

Volume / Issue

  • 20 / 3

Start / End Page

  • 842 - 846

PubMed ID

  • 352962

Pubmed Central ID

  • 352962

International Standard Serial Number (ISSN)

  • 0019-9567

Language

  • eng

Conference Location

  • United States