Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays.
Journal Article (Journal Article)
Nucleocapsids in quantities approaching 1 mg were purified from 109 measles virus-infected cells. They contained one polypeptide species with a molecular weight of 59,000. Antiserum was raised in rabbits against purified nucleocapsids and used in a competitive radioimmunoassay. Because of their instability, purified nucleocapsids were not suitable for use in such an assay. Instead partially purified nucleocapsids from HEp-2 cells persistently infected with measles virus and labeled in vitro with [35S]methionine were used as the source of radioactive antigen. The radioimmunoassay thus developed measured less than 5 ng of nucleocapsids in infected cells.
Full Text
Duke Authors
Cited Authors
- Moore, PM; Hayes, EC; Miller, SE; Wright, LL; Machamer, CE; Zweerink, HJ
Published Date
- June 1978
Published In
Volume / Issue
- 20 / 3
Start / End Page
- 842 - 846
PubMed ID
- 352962
Pubmed Central ID
- 352962
International Standard Serial Number (ISSN)
- 0019-9567
Digital Object Identifier (DOI)
- 10.1128/IAI.20.3.842-846.1978
Language
- eng
Conference Location
- United States