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Identification of viral infection by confocal microscopy.

Publication ,  Journal Article
Howell, DN; Miller, SE
Published in: Methods Enzymol
1999

Confocal microscopy is a valuable adjunct to electron microscopy in the fields of diagnostic and investigative virology. Confocal imaging can be used to examine large amounts of tissue stained by a variety of methods for evidence of viral infection. Areas thus identified can then be processed for ultrastructural study, allowing a highly focused search for viral pathogens. With the possible exception of the vibrating microtome, all of the equipment and reagents necessary for the preparation of specimens for confocal scanning are available in any well-stocked histology laboratory. Although originally developed to facilitate viral diagnosis by EM, the methods described herein can be applied to the ultrastructural study of any focal pathologic process.

Duke Scholars

Published In

Methods Enzymol

DOI

ISSN

0076-6879

Publication Date

1999

Volume

307

Start / End Page

573 / 591

Location

United States

Related Subject Headings

  • Viruses
  • Microscopy, Electron
  • Microscopy, Confocal
  • Mice
  • Liver
  • Indoles
  • Humans
  • Histocytochemistry
  • Fluorescent Dyes
  • Encephalitis, Viral
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Howell, D. N., & Miller, S. E. (1999). Identification of viral infection by confocal microscopy. Methods Enzymol, 307, 573–591. https://doi.org/10.1016/s0076-6879(99)07034-2
Howell, D. N., and S. E. Miller. “Identification of viral infection by confocal microscopy.Methods Enzymol 307 (1999): 573–91. https://doi.org/10.1016/s0076-6879(99)07034-2.
Howell DN, Miller SE. Identification of viral infection by confocal microscopy. Methods Enzymol. 1999;307:573–91.
Howell, D. N., and S. E. Miller. “Identification of viral infection by confocal microscopy.Methods Enzymol, vol. 307, 1999, pp. 573–91. Pubmed, doi:10.1016/s0076-6879(99)07034-2.
Howell DN, Miller SE. Identification of viral infection by confocal microscopy. Methods Enzymol. 1999;307:573–591.
Journal cover image

Published In

Methods Enzymol

DOI

ISSN

0076-6879

Publication Date

1999

Volume

307

Start / End Page

573 / 591

Location

United States

Related Subject Headings

  • Viruses
  • Microscopy, Electron
  • Microscopy, Confocal
  • Mice
  • Liver
  • Indoles
  • Humans
  • Histocytochemistry
  • Fluorescent Dyes
  • Encephalitis, Viral